Copolymers for capillary gel electrophoresis

Details Copolymers for capillary gel electrophoresis Copolymers for capillary gel electrophoresis

2005-08-09

2005-08-09

Noguerola, Alex (Department: 1753)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S605000

Reexamination Certificate

active

06926815

ABSTRACT:
This invention relates to an electrophoresis separation medium having a gel matrix of at least one random, linear copolymer comprising a primary comonomer and at least one secondary comonomer, wherein the comonomers are randomly distributed along the copolymer chain. The primary comonomer is an acrylamide or an acrylamide derivative that provides the primary physical, chemical, and sieving properties of the gel matrix. The at least one secondary comonomer imparts an inherent physical, chemical, or sieving property to the copolymer chain. The primary and secondary comonomers are present in a ratio sufficient to induce desired properties that optimize electrophoresis performance. The invention also relates to a method of separating a mixture of biological molecules using this gel matrix, a method of preparing the novel electrophoresis separation medium, and a capillary tube filled with the electrophoresis separation medium.

REFERENCES:
patent: 4048377 (1977-09-01), Boschetti et al.
patent: 4254249 (1981-03-01), Cottrell et al.
patent: 4725343 (1988-02-01), Hjerten et al.
patent: 4844786 (1989-07-01), Sugihara et al.
patent: 4874490 (1989-10-01), Hochstrasser
patent: 4877510 (1989-10-01), Chen
patent: 4948480 (1990-08-01), Christy, Jr. et al.
patent: 4963243 (1990-10-01), Ogawa et al.
patent: 4997537 (1991-03-01), Karger et al.
patent: 5006210 (1991-04-01), Yueng et al.
patent: 5055517 (1991-10-01), Shorr et al.
patent: 5069766 (1991-12-01), Zhu et al.
patent: 5149416 (1992-09-01), Osterhoudt et al.
patent: 5164055 (1992-11-01), Dubrow
patent: 5545302 (1996-08-01), Zhu et al.
patent: 5552028 (1996-09-01), Madabhushi et al.
patent: 5567292 (1996-10-01), Madabhushi et al.
patent: 5569364 (1996-10-01), Hooper et al.
patent: 5728282 (1998-03-01), Bashkin et al.
patent: 5759369 (1998-06-01), Menchen et al.
patent: 5785832 (1998-07-01), Chiari et al.
patent: 5885432 (1999-03-01), Hooper et al.
patent: 6013359 (2000-01-01), Takano et al.
patent: 6117293 (2000-09-01), Zhang et al.
patent: 6464850 (2002-10-01), Zhang et al.
patent: 0 324 539 (1989-01-01), None
patent: 0 339 678 (1989-04-01), None
patent: 0 340 653 (1989-04-01), None
Hirokazu Sawada & Kiyokatsu Jinno “Capillary electrophoretic separation of structurally similar solutes in noncross-linked poly(acrylamide-co-isopropylacrylamide solutin” Electrophoresis (1997) vol. 18, pp. 2030-2035.
Hirokazu Sawada & Kiyokatsu Jinno “Capillary electrophoresis with linear polymers containing Hydrophobic groups for the separation of small molecules” Analyst (1998), vol. 123, pp. 1471-1476.
Separation of polyelectrolytes of variable compositions by free-zone capillary electrophoresis; Journal of ChromatographyA, 766 (1997) pp. 233-236.
Separations of DNA fragments by capillary electrophoresis in N-substituted polyacrylamides; Journal of ChromatographyA, 781 (1977) pp. 347-355.
Multiplexed Fluorescence Detector for Capillary Electrophoresis Using Axial Optical Fiber Illumination; Analytical Chemistry, vol. 65 No. 7, Apr. 1, 1993 pp. 958-960.
Simultaneous Monitoring of DNA Fragments Separated by Electrophoresis in a Multiplexed Array of 100 Capillaries; Analytical Chemistry, vol. 66 No. 9, May 1, 1994; pp. 1424-1431.
High Speed DNA Sequencing by Using Mixed Poly(ethylene oxid ) Solutions in Uncoated Capillary Columns; Analytical Chemistry, vol. 6 No. 13, Jul. 1, 1995; pp. 1913-1919.

Affiliated with

Also associated with

Rating

Copolymers for capillary gel electrophoresis does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Copolymers for capillary gel electrophoresis, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Copolymers for capillary gel electrophoresis will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3514923