Cooled microscope slide and electrode apparatus for use in live

Details Cooled microscope slide and electrode apparatus for use in live Cooled microscope slide and electrode apparatus for use in live

1984-07-27

1985-12-31

Metz, Andrew H.

Chemistry: electrical and wave energy

Apparatus

Coating, forming or etching by sputtering

204302, 204308, 204274, 204289, B01D 5702

Patent

active

045619611

ABSTRACT:
A glass slide has parallel tubular electrodes for performing electro cell fusion. Fluid may be pumped through the electrodes to dissipate heat to enhance the yield of viable hybrids. An alternate embodiment sandwiches a gasket and parallel tubular electrodes between glass slides to permit cell fusion in a closed sterile environment.

REFERENCES:
patent: 4326934 (1982-04-01), Pohl
patent: 4441972 (1984-04-01), Pohl
Chen, C. S. et al., "Biological Dielectrophoresis: The Behavior of Lone Cells in a Nonuniform Electric Field", Annals New York Acadamy of Sciences, vol. 238, pp. 176-185, (1974).
Crane, J. S. et al., "Use of Balanced-Cell Technique to Determine Properties of Single Yeast Cells", J. Biol. Phys., vol. 5, pp. 49-73, (1977).
Pohl, H. A. et al., "The Continuous Positive and Negative Dielectrophoresis of Microorganisms", J. Biol. Phys., vol. 9, pp. 67-86, (1981).
D. E. P. Systems, Inc., brochure (1983), p. 4, "`Open` and `Closed` Dielectrophoretic Fusion Slides".

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