Conversion of mannose to fructose

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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435874, 435233, 4352533, 435174, 435176, 435177, 435181, 435182, C12P 1902, C12N 1100, C12N 990, C12N 120

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050494945

ABSTRACT:
An economical method of converting mannose to fructose uses a mannose isomerase from Pseudomonas cepacia immobilized on an alumina containing polyethyleneimine crosslinked with an excess of glutaraldehyde. The method utilizes mannose-containing aqueous solutions as the feedstock, and affords solutions in which at least 55% of the mannose has been converted to fructose. Because of the relatively higher levels of fructose than can be obtained by isomerizing glucose to fructose using glucose isomerase, substantial savings in separation of high fructose-containing products can be achieved. The process described represents the first economical mannose isomerase process.

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