Textiles: fiber preparation
Patent
1974-06-28
1976-08-03
Monacell, A. Louis
Textiles: fiber preparation
195100, 195115, C12D 1302, C12B 308
Patent
active
039727750
ABSTRACT:
A process for the production of sugar, mainly glucose, by the enzymatic degradation of cellulosic materials, particularly cellulosic wastes, which comprises hydrolyzing the cellulosic material in the presence of cellulase enzyme to produce a sugar solution and recovering from the hydrolysis products a major proportion of the cellulase enzyme used in the hydrolysis reaction for re-use. At least a portion of the required makeup cellulase enzyme is produced in a two-stage operation wherein, in the first stage, a portion of the output sugar solution is utilized to grow a cellulase-secreting microorganism, and, in the second stage, cellulase enzyme formation is induced in the microorganism-containing culture medium by the addition of an appropriate inducer, such as a cellulosic material. Cellulase enzyme is precipitated from the culture liquid by the addition of an organic solvent material, such as a low molecular weight alkyl ketone or alcohol, and the cellulase precipitate is then fed to the hydrolysis reaction.
REFERENCES:
patent: 3462275 (1969-08-01), Bellamy
patent: 3616222 (1971-10-01), Dasinger
patent: 3642580 (1972-02-01), Ghose
patent: 3711392 (1973-01-01), Metzger
patent: 3734831 (1973-05-01), Hulme
patent: 3764475 (1973-10-01), Mandels et al.
patent: 3812013 (1974-05-01), Bellamy et al.
Mandels et al., "The Production of Cellulose," Cellulases and Their Applications, Gould Ed. A.C.S. Publications Washington, D.C. (1969) pp. 391-396.
Mitra Gautam
Wilke Charles R.
Carlson Dean E.
Croft Irene S.
Monacell A. Louis
Robertson F. A.
The United States of America as represented by the United States
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