Drug – bio-affecting and body treating compositions – Whole live micro-organism – cell – or virus containing – Genetically modified micro-organism – cell – or virus
Patent
1995-05-09
1998-12-01
Naff, David M.
Drug, bio-affecting and body treating compositions
Whole live micro-organism, cell, or virus containing
Genetically modified micro-organism, cell, or virus
4351723, 435174, 435178, 435377, 435382, 435395, 424 937, 424422, A61K 4800, C12N 1100, C12N 500, C12N 1110
Patent
active
058434313
ABSTRACT:
Methods and compositions are provided for controlling cell distribution within an implantable bioartificial organ by exposing the cells to a treatment that inhibits cell proliferation, promotes cell differentiation, or affects cell attachment to a growth surface within the bioartificial organ. Such treatments include (1) genetically manipulating cells, (2) exposing the cells to a proliferation-inhibiting compound or a differentiation-inducing compound or removing the cells from exposure to a proliferation-stimulating compound or a differentiation-inhibiting compound; exposing the cells to irradiation, and (3) modifying a growth surface of the bioartificial organ with extracellular matrix molecules, molecules affecting cell proliferation or adhesion, or an inert scaffold, or a combination thereof. These treatments may be used in combination. Cells can be transformed with a proliferation-promoting gene such as the oncogene, SV40, linked to a regulatable promoter such as the Mx1 promoter, the promotor is activated in vitro to express the gene to result in cell proliferation, and the promotor is inactivated before or after insertion of the cells in the bioartificial organ to inhibit expression of the gene to reduce or stop cell proliferation in vivo. The promoter can be reactivated in vivo to again express the gene to result in further cell proliferation. The gene may be a proliferation-suppressing gene such as p53 gene or RB gene, or a differentiation-inducing gene such as high mobility group chromosomal protein 14. Inhibiting gene expression in vitro causes cell proliferation, and inducing gene expression reduces or stops cell proliferation in vivo.
REFERENCES:
patent: 4391909 (1983-07-01), Lim
patent: 4402694 (1983-09-01), Ash et al.
patent: 4489796 (1984-12-01), Kallok
patent: 4495288 (1985-01-01), Jarvis, Jr. et al.
patent: 4829000 (1989-05-01), Kleinman et al.
patent: 5002661 (1991-03-01), Chick et al.
patent: 5049493 (1991-09-01), Khosa et al.
patent: 5082670 (1992-01-01), Gage et al.
patent: 5106627 (1992-04-01), Aebischer et al.
patent: 5156844 (1992-10-01), Aebischer et al.
patent: 5158881 (1992-10-01), Aebischer et al.
patent: 5182111 (1993-01-01), Aebischer et al.
patent: 5250414 (1993-10-01), Schwab et al.
patent: 5283187 (1994-02-01), Aebischer et al.
patent: 5284761 (1994-02-01), Aebischer et al.
Farghali et al., The concept of Applicaiton of Immobilized and Perfused Mammalian cells (a bioreactor model) in biomedical research, Physiological Research, 43, pp. 117-120 (1994).
Adhya, Sankar, et al., "Promoter resurrection by activators--a minireview," Gene, 132, pp. 1-6 (1993).
Arnheiter, Heinz, et al., "Transgenic Mice with Intracellular Immunity to Influenza Virus," Cell, 62, pp. 51-61 (1990).
Aulthouse, Amy Lynn, et al., "Expression of the Human Chondrocyte Phenotype In Vitro," In Vitro Cellular & Developmental Biology, 25, pp. 659-658 (1989).
Baetge, E. Edward, et al., "Complete Nucleotide and Deduced Amino Acid Sequence of Bovine Phenylethanolamine N-methyltransferase: Partial Amino Acid Homology With Rat Tyrosine Hydroxylase," Proc. Natl. Acad. Sci. USA, 83, pp. 5454-5458 (1986).
Barinaga, Marcia, "Knockout Mice: Round Two," Science, 265, pp. 26-28 (1994).
Baron-Van Evercooren, A., et al., "Schwann Cell Differentiation in vitro: Extracellular Matrix Deposition and Interaction," Dev. Neurosci., 8, pp. 182-196 (1886).
Blau, Helen M., et al., "Myoblasts in Pattern Formation and Gene Therapy," Trends in Genetics, 9, pp. 269-274 (1993).
Bohak, Z., et al., "Novel Anchorage Matrices for Suspension Culture of Mammalian Cells," Biopolymers, 26, pp. S205-S213 (1987).
Brenner, Michael, et al., "GFAP Promoter Directs Astrocyte-specific Expression in Transgenic Mice," The Journal of Neuroscience, 14, pp. 1030-1037 (1994).
Brinster et al., "Factors Affecting the Efficiency of Introducing Foreign DNA into Mice by Microinjecting Eggs," Proc. Natl. Acad. Sci. USA, 82, pp. 4438-4442 (1985).
Cabasso, Israel, "Hollow-Fiber Membranes," Encyclopedia of Chemical Technology (Kirk-Othmer, ed.), pp. 492-517 (1980).
Carbonetto, Salvatore, "Ch. 15: Laminin Receptors: From PC12 Cells to PNS," Brain Repair (Bjorklund, Aguayo and Ottoson, eds.), pp. 185-197 (1990).
Celtrix Laboratories, "Vitrogen 100.RTM.: Purified Collagen for Cell Culture and Biochemistry" (Product Information Memorandum), Celtrix Laboratories, Palo Alto, CA (1991).
Cepko, Connie, "Retrovirus Vectors and Their Applications in Neurobiology," Neuron, 1, 345-453 (1988).
Chang, P.L., et al., "Delivery of Recombinant Gene Products with Microencapsulated Cells In Vivo," Human Gene Therapy, 4, pp. 433-440 (1993).
Chu, C.H., and A.M. Tolkovsky, "Alternative Adrenal Chromaffin Cell Fates Induced by Basic Fibroblast Growth Factor or Cyclic AMP In Vitro Depend on a Collaboration With The Growth Substrate," Neuroscience, 59, pp. 43-54 (1994).
Collaborative Research Incorporated, "CR-Laminin and CR-Anti-Laminin" (Product Information Memorandum), Collaborative Research, Inc., Bedford, MA (1987).
Collaborative Research Incorporated, "Basement Membrane Matrigel.TM." (Product Specification Sheet), Collaborative Research, Inc., Bedford, MA (1991).
Collagen Biomedical, "Collagen Test Implant Physician Package Insert," (Product Information Literature), Collagen Biomedical, Palo Alto, CA (1992).
Collagen Biomedical, "Zyderm.RTM. Collagen and Zyderm.RTM. Explained," (Product Information Memorandum), Collagen Biomedical, Palo Alto, CA (1992).
Collagen Biomedical, "Zyderm.RTM. Collagen Implant Physician Package Insert," (Product Literature), Collagen Biomedical, Palo Alto, CA (1992).
Collagen Biomedical, "Zyplast.RTM. Implant Physician Package Insert," (Product Literature), Collagen Biomedical, Palo Alto, CA (1992).
Crouch, Gary D., et al., "Ara-C Treatment Leads to Differentiation and Reverses the Transformed Phenotype in a Human Rhabdomyosarcoma Cell Line," Experimental Cell Research, 204, pp. 210-216 (1993).
Datta, Dipak B., "Keeping In Touch: The Adhesion Reactions of the Cell Membrane," A Comprehensive Introduction to Membrane Biochemistry, pp. 231-256 (1987).
de Bruine, Adriaan P., et al., "Extracellular Matrix Components Induce Endocrine Differentiation in Vitro in NCI-H716 Cells," American Journal of Pathology, 142, pp. 773-782 (1993).
De Loecker, William, et al., "Effects of Sodium Ascorbate (Vitamin C) and 2-Methyl-1,4-naphthoquinone (Vitamin K.sub.3) Treatment on Human Tumor Cell Growth in Vitro. II. Synergism with Combined Chemotherapy Action," Anticancer Research, 13, pp. 103-106 (1993).
Edgar, David, "Neuronal aminin receptors," TINS, 12, pp. 248-251 (1989).
El-Deiry, Wafik S., et al., "WAF1, a Potential Mediator of p53 Tumor Suppression," Cell, 75, pp. 817-825 (1993).
End, Peter, and Jurgen Engel, "Multidomain Proteins of the Extracellular Matrix and Cellular Growth," Receptors for Extracellular Matrix (McDonald, J. and Mecham,R., ed.), pp. 79-129 (1991).
Epstein-Baak, Ruth, et al., "Inducible Transformation of Cells from Transgenic Mice Expressing SV40 under lac Operon Control," Cell Growth and Differentiation, 3, pp. 127-134 (1992).
Fattaey, H.K., et al., "Modulation of Growth-Related Gene Expression and Cell Cycle Synchronization by a Sialoglycopeptide Inhibitor," Experimental Cell Research, 194, pp. 62-68 (1991).
Fattaey, Heideh, et al., "Inhibition of DNA Synthesis and Cell Division by a Cell Surface Sialoglycopeptide," Journal of Cellular Physiology, 139, pp. 269-274 (1989).
Fujiyama, C., et al., "Influence of Extracellular Matrix on the Proliferation and Differentiation of Adrenocortical Cells in Culture," Path. Res. Pract., 189, pp. 1205-1214 (1993).
Galli, Maria C., et al., "The Biology of Stem Cell Factor, a New Hematopoietic Growth Factor Involved in Stem Cell Regulation," Int. J. of Clin. Lab. Res., 23, pp. 70-77 (1993).
Gash, D.M., et al., "Amitotic Neuroblastoma Cells Used for Neural Implants in Monkeys," Science, 233, pp. 1420-1422 (1986).
Gonos, Efstathios S., and Demetrios A. Spandidos, "Oncogenes in Cellular Immortalisation and Differentiation (Review)," Anticancer Research, 13, pp. 1117-1122 (1993).
Grabham, Peter
Aebischer Patrick
Cain Brian M.
Doherty Edward J.
Gentile Frank T.
Hammang Joseph P.
Cytotherapeutics, Inc.
Elrifi Ivor R.
Naff David M.
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