Controlling cultivation conditions for animal cells

Details Controlling cultivation conditions for animal cells Controlling cultivation conditions for animal cells

1990-03-14

1994-04-19

Elliott, George C.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352401, 4352402, C12N 500, C12N 502, C12N 506

Patent

active

053044835

ABSTRACT:
Stable, long-term cultures of animal cells are accomplished by calculating the ratio of lactic acid formed by the cells to the glucose consumed by the cells (the L/G ratio) and controlling the cultivation conditions to maintain the L/G ratio between the limits of from greater than 0 to 0.6. When the ratio is outside the desired range, the dissolved oxygen concentration in the medium is increased and/or the glucose concentration in the medium is decreased.

REFERENCES:
Biotechnology And Bioengineering, vol. 27, No. 6, Jun., 1985, pp. 837-841. "Application of L-(+)-lactate electrode for clinical analysis and monitoring of tissue culture medium".
Journal Of Fermentation Technology, vol. 65, No. 3, 1987, "Effects of Lactate and Ammonium on the Oxygen Uptake Rate of Human Cells", T. Kimura et al, pp. 341-344.
Japan Journal Experimental Medicine, vol. 28, No. 2, 1958, "Establishment of Cell Strains of Rat Ascites Hepatoma Cells in Tissue Culture", T. Takaoka et al, pp. 115-127.
"Observations on the Carbohydrate Metabolism of Tumours", H. Crabtree, Laboratory Of The Imperial Cancer Research Fund, London, England, 1929.
Kagaku Kogaku Ronbunshu, 14, (3), 1988, M. Tokashiki et al, pp. 337-341.
Biotechnology And Bioengineering, vol. XXIII, "Development of a Hollow-Fiber System for Large-Scale Culture of Mammalian Cells", pp. 79-95; 1981.
Thomas "Nutrients, Oxygen, and pH" Chapter 5 in Mammalian Cell Technology, Thilly, (Editor), 1986, Butterworths Boston, London, Durban, Singapore, Sydney, Toronto, Wellington, pp. 109-130.
Paul "Carbohydrate And Energy Metabolism" Chapter 7 in Cells And Tissues In Culture: Methods, Biology And Physiology, vol. 1, Willmer, E. N. (Editor), 1965, Academic Press, London, New York pp. 239-276.
McKay, et al, Glucose Transport And Metabolism In Cultured Human Skin Fibroblasts Biochemica et Biophyska Acta vol. 762 pp. 198-204 1983.
Lehninger Biochemistry 2nd Edition pp. 244.gtoreq.245, 849 Worth Publishers, Inc. New York, N.Y. 1975.

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