Controlled translocation of a polymer in an electrolytic...

Details Controlled translocation of a polymer in an electrolytic... Controlled translocation of a polymer in an electrolytic...

2007-08-16

2010-06-08

Nguyen, Nam X (Department: 1795)

Chemistry: electrical and wave energy

Processes and products

Electrophoresis or electro-osmosis processes and electrolyte...

C204S547000, C204S400000, C204S403010, C205S775000, C435S006120, C435S287200

Reexamination Certificate

active

07731826

ABSTRACT:
An electrolytic sensing system for measuring a blocking signal allows for controlled translocation of a molecule, such as DNA, through a fluid channel. A substantially constant electric field supplied by a DC source is applied across the fluid channel and induces translocation of the molecule within the system. An oscillating electric parameter (e.g. current or voltage) supplied by an AC source is also applied across the fluid channel as a means for measuring a blocking signal. The substantially constant electric field can be altered to provide more detailed control of the molecule and, optionally, run a select portion of the molecule through the channel multiple times to provide numerous signal readings. A temperature control stage cools the system, providing further control of molecule translocation. A modified or non-modified protein pore may be utilized in the fluid channel. The system allows for long DNA strands to be sequenced quickly without amplification.

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Ying et al., “Frequency and Voltage Dependence of the Dielectrophoretic Trapping of Short Lengths of DNA and dCTP in a Nanopipette”, Biophysical Journal, vol. 86, pp. 1018-1027, Feb. 2004.

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