Controlled synthesis of peptide mixtures using mixed resins

Details Controlled synthesis of peptide mixtures using mixed resins Controlled synthesis of peptide mixtures using mixed resins

1990-05-15

1993-01-26

Lee, Lester L.

Chemistry: natural resins or derivatives; peptides or proteins;

Peptides of 3 to 100 amino acid residues

Synthesis of peptides

530333, C07K 100, C07K 104

Patent

active

051823667

ABSTRACT:
A method of preparing a mixture of peptides having a known composition and containing a peptide of a desired amino acid sequence is disclosed. The method involves three essential steps. First a given amount of a mixture of amino acyl or peptide derivatized resin is divided into a number of pools with each pool containing an equal molar amount of the resin mixture. Second a different single amino acid is coupled to the resin mixture in each of the pools and the coupling reaction is driven to completion. The peptide mixtures in each of the pools are then mixed together to obtain a complex peptide mixture containing each peptide in retrievable and analyzable amounts. The steps can be repeated to lengthen the peptide chains and methods can be employed to retrieve the desired peptide from the mixture and carry out analyses such as the determination of the amino acid sequence.

REFERENCES:
patent: 5010175 (1991-04-01), Rutter et al.
patent: 5053454 (1991-10-01), Judd
Houghten, R., Proc. Natl. Acad. Sci., 82:5131-5135, 1985.
Houghten, R. et al., Biotechniques, 4(6):522-524, 1986.
Genson, H. et al., Proc. Natl. Acad. Sci., 81: 3998-4002, 1984.

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