Controlled reversible poration for preservation of biological ma

Details Controlled reversible poration for preservation of biological ma Controlled reversible poration for preservation of biological ma

1998-09-11

2000-10-03

Saucier, Sandra E.

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of storing cells in a viable state

435 13, 435 2, C12N 500, A01N 102

Patent

active

061271772

ABSTRACT:
A preservation method for biological material having cell membranes includes reversibly porating the cell membranes; loading a bio-protective agent having bio-preservation properties to a predetermined intracellular concentration; preparing the bio-protective agent loaded biological material for storage; storing the biological material; recovering the stored biological material from storage; and reversing the cell membrane poration. H5 .alpha.-toxin, a genetically engineered mutant of Staphylococcus aureus .alpha.-hemolysin, may be used as a porating agent. Non-permeating sugars such as trehalose and sucrose may be used as the bio-protective agent.

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