Controlled reversible poration for preservation of...

Details Controlled reversible poration for preservation of... Controlled reversible poration for preservation of...

2005-06-07

2005-06-07

Afremova, Vera (Department: 1651)

Chemistry: molecular biology and microbiology

Animal cell, per se ; composition thereof; process of...

Method of storing cells in a viable state

C435S002000, C435S001300

Reexamination Certificate

active

06902931

ABSTRACT:
A preservation method for biological material having cell membranes includes reversibly porating the cell membranes; loading a bio-protective agent having bio-preservation properties to a predetermined intracellular concentration; preparing the bio-protective agent loaded biological material for storage; storing the biological material; recovering the stored biological material from storage; and reversing the cell membrane poration. H5 α-toxin, a genetically engineered mutant ofStaphylococcus aureusα-hemolysin, may be used as a porating agent. Non-permeating sugars such as trehalose and sucrose may be used as the bio-protective agent.

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