Controlled regeneration of zea diploperennis hybrids and breedin

Details Controlled regeneration of zea diploperennis hybrids and breedin Controlled regeneration of zea diploperennis hybrids and breedin

1987-02-11

1989-03-07

Warren, Charles F.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

4352405, 43524054, C12N 500

Patent

active

048106496

ABSTRACT:
This invention provides a method for high frequency plant regeneration from somatic stem donor tissue of field grown Zea diploperennis, a diploid, perennial corn ancestor with high tillering capacity. This species is used as a parent in a maize improvement strategy to transfer the unique traits of high tillering and plantlet regeneration capacity into cultivated corn. After 3-4 subcultures of cultured somatic tissues on a primary medium, small callus fragments are transferred to a secondary medium devoid of the auxin, 2,4-D. After a few days, numerous shoots regenerate and develop into normal plantlets which are then separated and transferred to a tertiary medium for root development. The selection of somaclonal variants form cultured somatic cells of interspecific hybrids between corn and teosinte are used for the synthesis of unique breeding lines suited for development of improved corn varieties. A protocol for gene transfer employing recombinant DNA techniques is also described.

REFERENCES:
patent: 4659668 (1987-04-01), Sondahl et al.
patent: 4677246 (1987-06-01), Armond et al.
"Maize and Cereal Protoplasts. Facts and Perspectives", Harms, C. T. In: Maize for Biological Research, pp. 373-384(1982).
"Plant Cell Culture and Somatic Cell Genetics of Cereals and Grasses", Vasil, I. K., Plant Improvement and Somatic Cell Genetics, pp. 179-203(1982).
"Chromosomal Variation and Frequency of Spontaneous Mutation Associated with In Vitro Culture and Plant Regeneration in Maize", Edallo, S., et al. Maydica 26:39-56(1981).
"Plant Regeneration from Tissue Cultures of Maize", Green, C. E. and R. L. Phillips, Crop. Sci., 15:417-421(1975).
"Proliferation and Plant Regeneration from the Nodal Region of Zea Mays L. (Maize, Gramineae) Embryos", Vasil, V., et al., Amer. J. Bot. 70(6):951-954(1983).
"Tissue Culture of Maize. III Plantlet Regeneration from Scutellar Callus" Barthkowicak, E., Genetica Polonica, 23(3):93-101 (1981).
"Tissue Culture Regeneration of Plants in Zea Dipoloperennis, a Close Relative of Corn", Sondahl, M. R., et al., Bio/Technology, May:455-458(1984).

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