Controlled regeneration of corn plants and breeding strategies t

Details Controlled regeneration of corn plants and breeding strategies t Controlled regeneration of corn plants and breeding strategies t

1984-04-06

1987-04-21

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435241, 47 58, C12N 500, C12N 502, A01B 7900

Patent

active

046596682

ABSTRACT:
This invention provides a method for high frequency plant regeneration from somatic stem donor tissue of field grown Zea diploperennis, a diploid, perennial corn ancestor with high tillering capacity. This species is used as a parent in a maize improvement strategy to transfer the unique traits of high tillering and plantlet regeneration capacity into cultivated corn. After 3-4 subcultures of cultured somatic tissues on a primary medium, small callus fragments are transferred to secondary medium devoid of the auxin, 2,4-D. After a few days, numerous shoots regenerate and develop into normal plantlets which are then separated and transferred to a tertiary medium for root development. The selection of somaclonal variants form cultured somatic cells of interspecific hybrids between corn and teosinte are used for the synthesis of unique breeding lines suited for development of improved corn varieties. A protocol for gene transfer employing recombinant DNA techniques is also described.

REFERENCES:
Prioli et al 1984 "Tissue Culture and Plant Regeneration in Diploid Perennial Teosinte" J Plant Phys v117 pp. 185-190.
Conger, (Editor) Cloning Agricultural Plants Via In Vitro Techniques CRC Press, 1981 pp. 16, 175.
Evans et al., (Editor) Handbook of Plant Cell Culture, vol. 1 Macmillan Publ. Co. 1983, p. 213.
Iltis et al., 1979 "Zea diploperennis(Gramineae: A New Teosinte from Mexico" Science v 203 pp. 186-188.
Allard 1960 "Principles of Plant Breeding" John Wiley & Sons p. 436.
Hackh's Chemical Dictionary, 4th Ed. 1971 p. 454.

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