Controlled oxidation of microbially produced cysteine-containing

Details Controlled oxidation of microbially produced cysteine-containing Controlled oxidation of microbially produced cysteine-containing

1984-10-17

1985-07-23

Schain, Howard E.

Chemistry of carbon compounds

Miscellaneous organic carbon compounds

C-metal

2601125R, 424 85, 424 88, 435 68, 435811, C07G 700, A61K 4502

Patent

active

045307873

ABSTRACT:
Method of oxidizing reduced cysteine-containing microbially produced synthetic proteins, such as synthetic IFN-.beta. or synthetic IL-2, in a controlled manner so that the synthetic proteins have the same disulfide bridging as their native counterparts. The oxidation employs o-iodosobenzoate as oxidizing agent and is carried out in an aqueous medium at a pH at least about one-half pH unit less than the pK.sub.a of the cysteines to be oxidized, a synthetic protein concentration of less than about 5 mg/ml, and an oxidizing agent:protein mol ratio that is at least stoichiometric, provided that the oxidizing agent is in excess in the terminal portion of the reaction.

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