Controlled modification of eukaryotic genomes

Details Controlled modification of eukaryotic genomes Controlled modification of eukaryotic genomes

1998-01-13

1999-06-08

Ketter, James

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving nucleic acid

4351723, 4353201, 435410, 536 231, 800205, C12Q 168, C12N 1582, C07H 2104, A01H 400

Patent

active

059104155

ABSTRACT:
A method of using a unique DNA construct for the creation of transgenic eukaryotic cells is described. The method allows a more precise and effective transformation procedure that targets the insertion of a DNA sequence into a predetermined DNA locus, while enabling the removal of any randomly inserted DNA sequences that occur as a by product of known transformation procedures.

REFERENCES:
"Directed Excision of a Transgene From The Plant Genome", Russell, et al., Mol. Gen. Genet., (1992), 234:49-59.
"Homologous Recombination Between Plasmid DNA Molecules in Maize Protoplasts", Lyznik, et al., Mol. Gen. Genet., (1991), 230:209-218.
"The FLP Recombinase of Yeast Catalyzes Site-Specific Recombination in the Drosophila Genome", Golic and Lindquist, Cell vol. 59, Nov. 3, 1989, 499-509.
"Recombinase-Mediated Gene Activation and Site-Specific Integration in Mammalian Cells", O'Gorman, et al., Reports, Mar. 15, 1991, 1351-1355.
"Development of a Heat Shock Inducible Expression Cassette for Plants: Characterization of Parameters for Its Use in Transient Assays", Ainley and Key, Plant Molecular Biology, 1990, 14: 949-967.
"Gene Transfer With Subsequent Removal of the Selection Gene From The Host Gene", Dale and Ow, Proc. Natl. Acad. Sci. USA, vol. 88, Dec. 1991, pp. 10558-10562.
"Exchange of Gene Activity in Transgenic Plants Catalyzed by the Cre-Iox Site Specific Recombination System", Bayley, et al., Plant Molecular Biology, 18: 353-361, 1992.
"Maize Polyubiquitin Genes: Structure, Thermal Perturbation of Expression and Transcript Splicing, and Promoter Activity Following Transfer to Protoplasts by Electroporation", Christensen, et al., Plant Molecular Biology, 18:675-689, 1992.
"A Steroid-Inducible Gene Expression System for Plant Cells", Schena, et al., Proc. Natl. Acad. Sci. USA, vol. 88, pp. 10421-10425, Dec. 1991.
"Selection-Gene-Free Transgenic Plants", NTIS Patent Application Serial No. 07/725,320, Filed Jul. 8, 1991, by U.S. Department of Agriculture, Washington, D.C. (PB92-10550).
"Site-directed recombination in the genome of transgenic tobacco", Odell et al., Mol. Gen. Gent., 223:369-378, Sep., 1990.
"The role of the IoxP spacer region in P1 site-specific recombination", Hoess et al., Nucleic Acid Research, vol. 14, No. 5, pp. 2287-2300, 1986.
"Transformation of Maize Cells and Regeneration of Fertile Transgenic Plants", Gordon-Kamm, The Plant Cell, vol. 2, pp. 603-618, Jul. 1990.
"Altering the Genome by Homologous Recombination", Capecchi, Science, vol. 244, pp. 1288-1292, 1989.
"Gene Targeting in Plants", Paszkowski et al., The EMBO Journal, vol. 7, No. 13, pp. 4021-4026, 1988.

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