Chemistry: electrical and wave energy – Processes and products – Electrostatic field or electrical discharge
Patent
1990-10-18
1992-04-28
Niebling, John
Chemistry: electrical and wave energy
Processes and products
Electrostatic field or electrical discharge
2041833, 204299R, G01N 2726
Patent
active
051085682
ABSTRACT:
A method of reducing electrophoretic mobility of macromolecules, particles, cells and other substances is provided which comprises interacting in a conventional electrophoretic separating procedure the substances with a polymer-linked affinity compound comprised of a hydrophilic neutral polymer such as polyethylene glycol bound to a second component such as a hydrophobic compound, an immunocompound such as an antibody or antibody active fragment, or a ligand such as a hormone, drug, antigen or a hapten. The reduction of electrophoretic mobility achieved is directly proportional to the concentration of the polymer-linked affinity compound employed, and such reduction can comprise up to 100% for particular particles and cells. The present invention is advantageous in that electrophoretic separation can now be achieved for substances whose native surface charge structure had prevented them from being separated by normal electrophoretic means. Depending on the affinity component utilized, separation can be achieved on the basis of specific/irreversible, specific/reversible, semi-specific/reversible, relatively nonspecific/reversible, or relatively nonspecific/irreversible ligand-substance interactions.
REFERENCES:
patent: 3984533 (1976-10-01), Uzgiris
patent: 4011044 (1977-03-01), Uzgiris
patent: 4102990 (1978-08-01), Uzgiris
patent: 4181589 (1980-01-01), Brooks
patent: 4783419 (1988-11-01), Hayashi et al.
Muller, W. et al., "Polyethylene Glycol Derivatives of Base and Sequence Specific DNA Ligands: DNA Interaction and Application for Base Specific Separation of DNA Fragments by Gel Electrophoresis", Nucleic Acids Research, vol. 9, No. 1, (1981), pp. 95-119.
Broad, Jr. Robert L.
Manning John R.
Niebling John
Seemann Jerry L.
Starsiak Jr. John S.
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