Controlled hydrodynamic cell culture environment for three dimen

Details Controlled hydrodynamic cell culture environment for three dimen Controlled hydrodynamic cell culture environment for three dimen

1998-05-22

1999-12-14

Beisner, William H.

Chemistry: molecular biology and microbiology

Apparatus

Bioreactor

4353042, 4352882, 435394, C12M 302

Patent

active

060016434

ABSTRACT:
A novel hydrodynamic cell culture environment is disclosed for a two-chamber roller bottle. The unstable hydrodynamics of a gas headspace media chamber is coupled through a center opening to a second zero headspace culture chamber. Coupling the two chambers creates novel fluid streamlines that add turbulence for improved mass transfer and slow media repletion. The exchange between chambers regulates turbulence and thus concomitant exchange of nutrients i.e., environmental control. It is disposable, automatable, and suited to culturing tissue for transplants.

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