Controlled gene excision

Details Controlled gene excision Controlled gene excision

1985-02-13

1988-05-10

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing alpha or beta amino acid or substituted amino acid...

435 68, 435 70, 4351723, 435253, 435320, 435849, 935 14, 935 42, 935 43, C12P 1322, C12P 2100, C12P 2102, C12N 1500, C12N 120, C12N 700

Patent

active

047435461

ABSTRACT:
An Escherichia coli chromosomal DNA segment is engineered for controllable excision and loss from the E. coli cell population. The DNA segment has a gene determining a function that is lost in the absence of that chromosomal segment. Specifically, the segment includes a pair of lambdoid phage att sites positioned at opposite ends thereof, and a gene encoding the function. The E. coli cell includes DNA encoding lambdoid phage int and xis, positioned for transcription under control of an external stimulus. By excising the chromosomal DNA segment once a desired cell population is achieved, cell growth and diversion of intermediates from a desired fermentation product are diminished or avoided.

REFERENCES:
Howard et al., in Experimental Manipulation of Gene Expression, Academic Press, 1983, pp. 137-153.
Murray et al., in Lambda II, Cold Spring Harbor Press, 1983, pp. 395-432.
Leplatois et al., (1983), Biochimie, 65:317-324.
Wilson et al., (1979), J. Mol. Biol., 132:471-491.
Murray et al., (1979), J. Mol. Biol., 132:493-505.
Struhl, (1981), J. Mol. Biol., 152:517-533.
Plasterk, (1983), Virol., 127:24-36.
Echols et al., "Control of Integration and Excision", Lambda II, (Hendrix et al., Ed.), CSH Laboratory, (1983), pp. 75-92.
Joyce et al., (1984), J. Bacteriol., 158:636-643.

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