Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1995-06-06
1999-07-20
Robinson, Douglas W.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
470DIG1, 4353201, 435419, 536 232, 536 2372, A01H 500, C12N 514, C12N 1533, C12N 1552, C12N 1582
Patent
active
059258063
ABSTRACT:
A novel compositions and methods useful for genetic engineering of plant cells to provide a method of controlling the timing or tissue pattern of expression of foreign DNA sequences inserted into the plant plastid genome are provided in the instant invention. Constructs include those for nuclear transformation which provide for expression of a viral single subunit RNA polymerase in plant cell tissues, and targeting of the expressed polymerase protein into the plant cell plastids. In addition, plastid expression constructs comprising a viral gene promoter region which is specific to the RNA polymerase expressed from the nuclear expression constructs described above, and a DNA sequence of interest to be expressed in the transformed plastid cells are provided. Plant cells and plants comprising the nuclear and/or plastid constructs described herein are of interest. Of particular interest is a method of controlling expression of the inserted plastid gene constructs in a tissue and/or developmental specific manner in plants comprising both the nuclear polymerase construct and the plastid expression constructs.
REFERENCES:
patent: 5409823 (1995-04-01), Crossland et al.
patent: 5576198 (1996-11-01), McBride et al.
Svab, et.al. "High-Frequency Plastid Transformation in Tobacco by Selection for a Chimeric aadA Gene" Proc. Natl. Acad. Sci, USA 1993 vol. 90 913-917.
Svab, et.al. "Stable Transformation of Plastids in Higher Plants" Proc. Natl. Acad. Sci, USA 1990 vol. 87 8526-8530.
Boynton, et. al. "Chloroplast Transformation in Chlamydomonas with High Velocity Microprojectiles" Science, 1988 vol. 240: 1534-1538.
Ohyama, et. al. "Chloroplast Gene Organization Deduced from Complete Sequence of Liverwort Marchantia polymorpha Chloroplst DNA" Nature 1986, vol. 322 572-574.
Hiratsuka, et. al. "The Complete Sequence of the Rice (Oryza sativa) Chloroplast Genome: Intermolecular Recombination Between Distinct tRNA Genes Accounts for a Major Plastid DNA Inversion During the Evolution of Cereals" Mol. Gen. Genet. 1989 vol. 217 185-194.
Shinozaki, et. al., "The Complete Nucleotide Sequence of the Tobacco Chloroplast Genome: its Gene Organization and Expression" The EMBO Journal 1986 vol. 5 No. 9 2043-2049.
Rosenberg, et. al "Vectors for Selective Expression of Cloned DNA's by T7 RNA Polymerase" Gene 1987 vol. 56 125-135.
Benton, et. al., "Signal-Mediated Import of Bacteriophage T7 RNA Polymerase into the Saccharomyces cerevisae Nucleus and Specific Transcription of Target Genes " Molecular and Cell Biology 1990 vol. 10 No. 1 353-360.
Lieber, et. al. "High Level Gene Expression in Mammalian Cells by a Nuclear T7-phage RNA Polymerase" Nucleic Acids Research 1989 vol. 7 No. 21 8485-8493.
Lassner, et. al. "Targeting of T7 RNA Polymerase in Tobacco Nuclei Mediated by an SV40 Nuclear Location Signal" Plant Molecular Biology 1991 vol. 17 229-234.
Gruissem, et. al "Control Mechanisms of Plastid Gene Expression" Critical Reviews in Plant Sciences 1993 vol. 12 1/2 19-55.
De Block, et. al., "Chloroplast Transformation by Agrobacterium tumefaciens" The EMBO Journal 1985 vol. 4 No. 6 1367-1372.
Boynton, et. al. "Manipulating the Chloroplast Genome of Chlamydomonas--Molecular Genetics and Transformation" Current Research in Photosynthesis Proceedings of the VIIth International Conference on Photosynthesis, Stockholm, Sweden, Aug. 6-11, 1989 vol. III 12.509-12.516.
Schinkel, et. al. "Mitochondrial RNA Polymerase: Dual Role in Transcription and Replication" TIG 1989 vol. 5, No. 5 149-153.
Masters, et. al., "Yeast Mitochondrial RNA is Homologous to Those Encoded by Bacteriophages T3 and T7" Cell, Oct. 9, 1987 vol. 51 89-99.
Daniell, et. al. "Transient Foreign Gene Expression of Chloroplasts of Cultured Tobacco Cells After Biolistic Delivery of Chloroplast Vectors" Proc. Natl. Acad. Sci, USA 1990 vol. 87 88-92.
McGraw, et. al. "Sequence and Analysis of the Gene for Bacteriophage T3 RNA Polymerase" Nucleic Acids Research 1985 vol. 13 No. 18 6753-6767.
Staub, et. al. "Accumulation of D1 Polypeptide in Tobacco Plastids is Regulated via the Untranslated Region of the psbA mRNA" The EMBO Journal, 1993 vol. 12 601-606.
Dunn, et. al. "Targeting Bacteriophage T7 RNA Polymerase to the Mammalian Cell Nucleus" Gene 1988 vol. 68 259-266.
O'Neal, et. al. "Isolation of Tobacco SSU Genes: Characterization of a Transcriptionally Active Pseudogene" Nucleic Acids Research 1987 vol. 15 No. 21 8661-8677.
McBride, et al., Controlled Expression of Plastid Transgenes in Plants Based on A Nuclear DNA-encoded and Plastid-targeted T7 RNA Polymerase.
Roy, et al. (1988) Nucleic Acids Research 16(24): 11759-11767.
Gruissem, et al. (1993) Critical Reviews in Plant Sciences 12 1/2: 19-55.
Lassner, et al. (1991) Plant Molecular Biology 17:229-234.
Lieber, et al (1989) Nucleic Acids Research 17(21): 8485-8493.
Cashmore, et al. (1985) Bio/Technology 3: 803-808.
McBride Kevin E.
Stalker David M.
Nelson Amy J.
Robinson Douglas W.
LandOfFree
Controlled expression of transgenic constructs in plant plastids does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Controlled expression of transgenic constructs in plant plastids, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Controlled expression of transgenic constructs in plant plastids will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1323259