Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1993-12-10
1996-07-09
Ulm, John
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
4351723, 536 241, C12N 1567
Patent
active
055344186
ABSTRACT:
The present invention provides methods for the controlled production of recombinant proteins in cells. Cells employed in the invention method contain a gene encoding the desired recombinant protein, with transcription of the gene maintained under the control of a transcriptional control element which is activated by a ligand/receptor complex. The ligand/receptor complex is formed when a ligand (which is a hormone or/and analog thereof) is complexed with a receptor (which is a hormone receptor or functional analog thereof which has the transcription activating properties of the receptor). Receptor is produced by the expression of non-endogenous DNA which is also present in the cells used for production of recombinant protein.
REFERENCES:
patent: 4859585 (1989-08-01), Sonnenschein et al.
patent: 4859609 (1989-08-01), Dull et al.
Cell 33:489-99, Jun. 1983, Chandler et al DNA Sequence, Bound Specifically by, Glucocorticoid Receptor In Vitro Render A Heterologous Promoter . . . .
J. Biol. Chem. 258:12693-701, 25 Oct. 1983, Lai et al Regulated Expression of the Chicken Ovalbumin Gene in Human Estrogen responsive Cell Line.
Parks et al., "Dexamethasone Stimulation of Murine Mammary Tumor Virus Expression: A Tissue Culture Source of Virus", Science vol. 184:158-160 (1974).
Ringold et al., "Dexamethasone-Mediated Induction of Mouse Mammary Tumor Virus RNA: a system for studying Gluccocorticoid Action", Cell vol. 6:299-305 (1975).
Kurtz and Feigelson, "Multihormonal induction of hepatic alpha.sub.2u -globulin mRNA as measured by hybridization to complementary DNA", Proc. Natl. Acad. Sci. USA vol. 74:4791-4795 (1977).
Krust et al., "The chicken oestrogen receptor sequence: homology with v-erbA and the human oestrogen and glucocorticoid receptors", The EMBO Journal vol. 5:891-897 (1986).
Danielsen et al., "The mouse glucocorticoid receptor: mapping of functional domains by cloning, sequencing and expression of wild-type and mutant receptor proteins", the EMBO Journal vol. 5:2513-2522 (1986).
Northrop et al., "Analysis of Glucocorticoid Unresponsive Cell Variants Using a Mouse Glucocorticoid Receptor Complementary DNA clone", The Journal of Biological Chemistry vol. 261:11064-11070 (1986).
Miesfeld et al., "Characterization of a steroid hormone receptor gene and mRNA in wild-type and mutant cells", Nature vol. 312:779-781 (1984).
Weinberger et al., "Identification of Human Glucocorticoid Receptor Complimentary DNA Clones by Epitope Selection", Science vol. 228:740-742 (1985).
Green et al., "Human oestrogen receptor cDNA: sequence, expression and homology to v-erb-A", Nature vol. 320:134-139 (1986).
Evans et al., "Glucocorticoid and thyroid hormones transcriptionally regulate growth hormone gene expression", Proc. Nat. Acad. Sci. USA vol. 79:7659-7663 (1982).
Spindler et al., "Growth Hormone Gene Transcription Is Regulated by Thyroid and Glucocorticoid Hormones in Cultured Rat Pituitary Tumor Cells", The Journal of Biological Chemistry vol. 257:11627-11632 (1982).
Narayan et al., "Rapid induction of a specific nuclear mRNA precursor by thyroid hormone", Proc. Natl. Acad. Sci. USA vol. 81:4687-4691 (1984).
Karin et al., "Metallothionein mRNA induction in HeLa cells in response to zinc or dexamethasone is a primary induction response" Nature vol. 286:295-297 (1980).
Hager and Palmiter, "Transcriptional regulation of mouse liver metallothionein-I gene by glucocorticoids", Nature vol. 291:340-342 (1981).
Robins et al., "Regulated Expression of Human Growth Hormone Genes in Mouse Cells", Cell vol. 29:623-631 (1982).
Arriza Jeffrey
Evans Roland M.
Giguere Vincent
Hollenberg Stanley M.
Ong Estelita S.
Reiter Stephen E.
The Salk Institute for Biological Studies
Ulm John
LandOfFree
Controlled expression of recombinant proteins does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Controlled expression of recombinant proteins, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Controlled expression of recombinant proteins will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1866624