Controlled environment vitrification system for preparation of l

Details Controlled environment vitrification system for preparation of l Controlled environment vitrification system for preparation of l

1986-08-07

1988-06-28

Scott, Samuel

Chemistry: molecular biology and microbiology

Apparatus

Including measuring or testing

435290, 62 64, C12M 100

Patent

active

047538872

ABSTRACT:
A system for preparing specimens in a controlled environment to insure that a liquid or partially liquid specimen is maintained in its original state while it is being prepared, and once prepared the specimen is vitrified or solidified with minimal alteration of its microstructure. The controlled environment is provided within a chamber where humidity and temperature can be controlled precisely while the specimen is prepared. The specimen is mounted on a plunger and a shutter controlled opening is opened substantially simultaneously with release of the plunger so the specimen is propelled through the shutter into an adjacent cryogenic bath.

REFERENCES:
patent: 4090921 (1978-05-01), Sawamura et al.
patent: 4209585 (1980-06-01), Lloyd et al.
patent: 4304293 (1981-12-01), Scheiwe et al.
patent: 4489569 (1984-12-01), Sitte
patent: 4578963 (1986-04-01), Sitte
patent: 4580416 (1986-04-01), Sitte
patent: 4676070 (1987-06-01), Linner
patent: 4680945 (1987-07-01), Hoffmeister
Adrian et al., "Cryo-Electron Microscopy of Viruses", Nature, vol. 308, No. 5954, pp. 32-36 (Mar. 1984).
Reichert Scientific Instruments Division of Warner Lambert Technologies Inc., Model KF80 Immersion Cryofixation System according to Sitte.

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