Chemistry: molecular biology and microbiology – Apparatus – Bioreactor
Patent
1980-04-04
1981-11-17
Warden, Robert J.
Chemistry: molecular biology and microbiology
Apparatus
Bioreactor
119 37, 435298, 435809, C12M 138
Patent
active
043012523
ABSTRACT:
A miniature, biological incubator for continuous light microscopic observation of cells in cell cultures under environmentally controlled conditions for use in conjunction with petri dishes or similar types of culture dishes, in which different covers are provided to accommodate different types of microscopes and magnification, the incubator being small enough to fit on a microscope stage and to have a sufficiently small internal volume to facilitate maintenance of a controlled atmosphere having stable temperature, humidity and mixture of gases therein and to permit rapid restoration of controlled environment lost during replacement of dishes or during changing of covers to suit particular microscopic observation. The chamber contains a means for humidifying the gases contained therein by evaporation from water-saturated absorbent pads of large surface area, plus a means for humidifying particular gaseous mixtures used to purge the chamber, utilizing at least one hollow fiber tube in intimate contact with water-saturated absorbent pads through which the purging gases are passed, the hollow fiber tube presenting to the purging gases a large wetted surface area resulting in efficient humidification of the purging gases.
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Baker Fraser L.
Baumann John H.
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