Multicellular living organisms and unmodified parts thereof and – Plant – seedling – plant seed – or plant part – per se – Higher plant – seedling – plant seed – or plant part
Patent
1997-12-19
1999-07-20
Robinson, Douglas W.
Multicellular living organisms and unmodified parts thereof and
Plant, seedling, plant seed, or plant part, per se
Higher plant, seedling, plant seed, or plant part
800295, 435419, 4353201, 435468, 435469, 435470, 536 241, 536 236, 536 245, C12N 1500, C12N 1529, C12N 1582, A01H 400
Patent
active
059258080
ABSTRACT:
A method for making a genetically modified plant comprising regenerating a whole plant from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences, a second gene that encodes a recombinase specific for the specific excision sequences linked to a repressible promoter, and a third gene that encodes the repressor specific for the repressible promoter. Also a method for making a genetically modified hybrid plant by hybridizing a first plant regenerated from a plant cell that has been transfected with DNA sequences comprising a first gene whose expression results in an altered plant phenotype linked to a transiently active promoter, the gene and promoter being separated by a blocking sequence flanked on either side by specific excision sequences to a second plant regenerated from a second plant cell that has been transfected with DNA sequences comprising a second gene that encodes a recombinase specific for the specific excision sequences linked to a promoter that is active during seed germination, and growing a hybrid plant from the hybrid seed. Plant cells, plant tissues, plant seed and whole plants containing the above DNA sequences are also claimed.
REFERENCES:
patent: 4959317 (1990-09-01), Sauer
patent: 5159135 (1992-10-01), Umbeck
patent: 5217889 (1993-06-01), Roninson et al.
patent: 5231019 (1993-07-01), Paszkowski et al.
patent: 5244802 (1993-09-01), Rangan
patent: 5270201 (1993-12-01), Richards et al.
patent: 5304730 (1994-04-01), Lawson et al.
patent: 5352605 (1994-10-01), Fraley et al.
patent: 5723765 (1998-03-01), Oliver et al.
Lanzer, Michael and Bujard, Hermann, "Promoters largely determine the efficiency of repressor action," Proc. Natl. Acad. Sci. USA, 85: 8973-8977 (1988).
Araki, Kimi et al., Site-specific recombination of a transgene in fertilized eggs by transient expression of Cre recombinase, Proc. Natl. Acad Sci USA 92(1) 1995.
Bayley, Christopher C., et al., Exchange of gene activity in transgenic plants catalyzed by the Cre-lox site-specific recombination system, Plant Molecular Biology, 18:353-361 (1992).
Medberry, Scott L., et al., Intra-chromosomal rearrangements generated by Cre-lox site-specific recombination, Nucleic Acids Research, 23(3):485-490 (1995).
Chapman, Sean, Kavanagh, Tony and Baulcombe, David, "Potato virus X as a vector for gene expression in plants," The Plant Journal, 2(4):549-557, 1992.
Odell, Joan T., et al., Seed-Specific Gene Activation Mediated by the Cre/lox Site-Specific Recombination System, Plant Physiol., 106:447-458 (Oct. 2, 1994).
Qin, Minmin, et al., Cre recombinase-mediated site-specific recombination between plant chromosomes, Proc. Natl. Acad. Sci. USA, 91:1706-1710 (1994).
Sauer, Brian, Manipulation of Transgenes by Site-Specific Recombination: Use of Cre Recombinase, Methods in Enzymology, 225:890-900 (1993).
Barinaga, Marcia, "Knockout mice: round two," Science, 265:26-28 (1994).
Gu, Hua, Marth, Jamey D., Orban, Paul C., Mossmann, Horst and Rajewsky, Klaus, "Deletion of a DNA of a polymerase B gene segment in T cells using cell type-specific gene targeting," Science, 265:103-106 (1994).
Geissendorfer and Hillen, "Regulated expression of heterologous genes in Bacillus subtilis using the Tn10 encoded tet regulatory elements," Appl. Microbiol. Biotechnol., 33:657-663 (1990).
Gatz and Quail, "Tn10-encoded tet repressor can regulate an operator-containing plant promoter," Proc. Natl. Acad. Sci. USA, 85:1394-1397 (1988).
Gatz, et al., "Regulation of a modified CaMV 35S promoter by the Tn10-encoded Tet repressor in transgenic tobacco," Mol. Gen. Genet., 227:229-237 (1991).
Gatz, et al., "Regulation of a plant promoter by a bacterial repressor protein in transgenic tobacco plants," (Abstract) Herbsttagung der Gesellschaft fur Biologische Chemie, 372:659-660 (1991).
Roder, et al., "Efficiency of the tetracycline-dependent gene expression system: complete suppression and efficient induction of the rolB phenotype in transgenic plants," Mol. Gen. Genet., 243:32-38 (1994).
Gatz, et. al., "Stringent repression and homogenous de-repression by tetracycline of a modified CaMV 35S promoter in intact transgenic tobacco plants," The Plant Journal, 2:397-404 (1992).
Rogers, S.G., Klee H.J., Horsch, R.B. and Fraley, R.T., "Improved vectors for plant transformation: expression cassette vectors and new selectable markers," Methods in Enzymology, 153:253-305 (1987).
Shen, Wen-Hui and Hohn, Barbara, "Excision of a transposable element from a viral vector introduced into maize plants by agroinfection," The Plant Journal, 2(1):35-42 (1992).
Pan, Guohua, Luetke, Karen and Sadowski Paul D., "Mechanism of cleavage and ligation by FLP recombinase: classification of mutations in FLP protein by in vitro complementation analysis," Molecular and Cellular Biology, 13(6):3167-3175 (1993).
Hall, Samantha C. and Halford, Stephen E., "Specificity of DNA recognition in the nucleoprotein complex for site-specific recombination by Tn21 resolvase," Nucleic Acids Research, 21(24):5712-5719 (1993).
Wilde, Robin J., Cooke, Susan E., Brammar, William J. and Schuch, Wolfgang, "Control of gene expression in plant cells using a 434:VP16 chimeric protein," Plant Molecular Biology, 24:381-388 (1994).
Muskhelishvili, Georgi, Palm, Peter and Zillig, Wolfram, "SSV1-encoded site-specific recombination system in Sulfolobus shibate," Mol. Gen Genet 237:334-342 (1993).
Sadowski, Paul D., "Site-specific genetic recombination: hops, flips, and flops," The FASEB Journal, 7:760-767 (1993).
Lloyd, Alan M., Davis, Ronald W., "Functional expression of the yeast FLP/FRT site-specific recombination system in Nicotiana tabacum," Mol. Gen. Genet., 242:653-657 (1994).
Horn, Mark A., Heinstein, Peter F. and Low, Philip S., "Biotin-mediated delivery of exogenous macromolecules into soybean cells," Plant Physiol., 93:1492-1496 (1990).
Mett, Vadim L., Lochhead, Leesa P. and Reynolds, Paul H.S., "Copper-controllable gene expression system for whole plants," Proc. Natl. Acad. Sci. USA, 90:4567-4571 (1993).
Schena, Mark, Lloyd, Alan M. and Davis, Ronald W., "A steroid-inducible gene expression system for plant cells," Proc. Natl. Acad. Sci. USA, 88:10421-10425 (1991).
Wilde, R.J., Shufflebottom, D., Cooke, S., Jasinska, I., Merryweather, A., Beri, R., Brammar, W.J., Bevan, M. and Schuch, W., Control of gene expression in tobacco cells using a bacterial operator--repressor system, The EMBO Journal, 11(4):1251-1259 (1992).
Hamamoto, Hiroshi, Sugiyama, Yoshinori, Nakagawa, Noriaki, Hashida, Eiji, Matsunaga, Yuji, Takemoto, Shizume, Watanabe, Yuichiro and Okada, Yoshimi, "A new tobacco mosaic virus vector and its use for the systemic production of angiotensin-I-converting enzyme inhibitor in transgenic tobacco and tomato," Biotechnology, 11:930-932 (1993).
Zou, Y.R., Muller, W., Raiewsky, K., Cre-loxP-mediated gene replacement: a mouse strain producing humanized antibodies. Current biology. Dec. 01, 1994 4(12):1099-1103.
Keim Don Lee
Oliver Melvin John
Quisenberry Jerry Edwin
Trolinder Norma Lee Glover
Delta and Pine Land Company
Haas Thomas
Robinson Douglas W.
The United States of America as represented by the Secretary of
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