Chemistry: natural resins or derivatives; peptides or proteins; – Proteins – i.e. – more than 100 amino acid residues – Separation or purification
Reexamination Certificate
2006-09-05
2006-09-05
Tate, Christopher (Department: 1654)
Chemistry: natural resins or derivatives; peptides or proteins;
Proteins, i.e., more than 100 amino acid residues
Separation or purification
C530S350000, C514S002600, C435S069100
Reexamination Certificate
active
07101982
ABSTRACT:
The invention provides methods of controlling pH variations during chromatography. In particular embodiments, the invention includes equilibrating and eluting proteins from chromatography resins have a solid phase that possesses a charged functional group on its backbone under controlled conditions of osmolarity and pH. The invention is particularly useful in the purification of proteins that are sensitive to pH transitions such as, for example, the IL-1 Receptor type II protein.
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Clayton and Bushuk, “Ion Exchange Chromatography of Proteins Artifact Separations Resulting From Inadequate pH Control,”J Chromatog21:67-74, 1966.
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Ghose Sanchayita
McNerney Thomas M.
Fowler Kathleen
Immunex Corporation
Tate Christopher
Teller Roy
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