Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Patent
1994-12-22
1997-02-04
Elliott, George C.
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
435 695, 435 6951, 435 6952, C12P 2102, C12P 2104
Patent
active
055996906
ABSTRACT:
Norleucine incorporation into recombinant gene products may be inhibited by culturing the cells expressing the gene products in media comprising a high level of leucine and/or methionine, or by expressing the gene products in a leu mutant. Norleucine incorporation in recombinant gene products may be promoted by culturing the cells expressing the gene products in media comprising norleucine or deficient in leucine and/or methionine. For example, norleucine analogs of IL-2, GCSF, .gamma.-interferon and .alpha.-consensus interferon may be produced in this way.
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Fenton Dennis
Lai Por-Hsiung
Lu Hsieng
Mann Michael
Tsai Larry
Amgen Inc.
Elliott George C.
Winter Robert B.
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