Drug – bio-affecting and body treating compositions – Designated organic active ingredient containing – Peptide containing doai
Patent
1993-11-08
1996-03-05
Feisee, Lila
Drug, bio-affecting and body treating compositions
Designated organic active ingredient containing
Peptide containing doai
530300, 530350, 530365, 530366, 530386, 5303871, 5303882, 530395, 530416, 530832, 530833, 530861, 530863, 530864, 514 12, 4241981, C07K 1400, C07K 1606, C07K 1618, A61K 3817
Patent
active
054968020
DESCRIPTION:
BRIEF SUMMARY
BACKGROUND OF THE INVENTION
1. Field of the invention
This invention relates to a newly isolated protein from cow's milk and the use of the protein or antibodies thereto for the control of milk secretion in lactating animals.
2. Description of the Prior Art
The rate of milk secretion by a lactating animal is regulated by the frequency of milk removal. In other words, there Is a mechanism which acts to match the animal's supply of milk to the demand of her offspring or of a farmer's milking regime. Part of this control is achieved by the release of galactopoietic hormones during suckling or milking. However, studies by workers at the Hannah Research Institute, Ayr, Scotland on lactating goats have shown that another factor is involved. This is an inhibitor which decreases milk secretion at a local level, i.e. at the individual gland of an udder.
It has already been shown that the inhibitor is present in a goat milk fraction containing whey proteins of molecular weight 10-30 KDa, this range of molecular weights being determined by the nominal sizes of filters used in ultrafiltration of the whey. The effect has been demonstrated both in vitro and in vivo. The in vitro technique, described by C. J. Wilde et al., Biochem. J. 242, 285-288 (1987), consists in culturing explanted pieces of rabbit mammary with and without the milk fraction and demonstrating the inhibition of lactose and casein synthesis. See also G. M. Stewart et al., J. Endocrinology 118, R1-R3 (1988). In the vivo technique, C. J. Wilde et al., Quarterly Journal of Experimental Physiology 73, 391-397 (1988), the milk fraction was injected into a single mammary gland of goats via the teat canal. A temporary dose-dependent reduction of milk yield, specific to that gland, was observed.
It has remained a problem to determine whether an inhibitor is present in cow's milk and, if so, to purify it sufficiently for identification, with a view to chemical or biological synthesis.
REFERENCE TO UNPUBLISHED PRIOR PATENT APPLICATIONS
Our unpublished U.K. Patent Application 9024653.9 entitled "CONTROL OF MILK SECRETION", filed 13th Nov. 1990, claiming priority of U.K. Patent Application 8925594.7 filed 13th Nov. 1989, describes and claims a protein isolated from the 10-30 KDa (nominal) fraction of whey proteins of goat's milk and having an inhibitory effect on milk secretion. This protein has a molecular weight (by gel filtration chromatography) of about 7.6 KDa and is present in the third significant peak when a said fraction is resolved on a defined anion exchange column under defined conditions. There are corresponding patent applications of the same date and priority in the PCT (GB90/01742), Ireland (90/4086) and New Zealand (236054).
Our companion unpublished U.K. Patent Application 9024649.7 entitled "CONTROL OF SECRETION OF MILK", filed 13th Nov. 1990, claiming priority of U.K. Patent Application No. 8925595.4 filed 13th Nov. 1989, describes and claims a protein isolated from the 10-30 KDa (nominal) fraction of whey proteins of cow's milk and having an inhibitory effect on milk secretion. This protein has a molecular weight (by gel filtration chromatography) of about 7 KDa and is present in the second significant peak (designated peak "2A") when a said fraction is resolved on a defined anion exchange column under defined conditions. There are corresponding patent applications of the same date and priority in the PCT (GB90/01743), Ireland (90/4087) and New Zealand (236051).
SUMMARY OF THE INVENTION
It has now been found that inhibitory activity towards milk secretion is also present in the eighth significant peak (6B) when a said fraction of cow's milk whey proteins is resolved under the same conditions as referred to above.
There are various ways of defining the protein of the invention, of varying degrees of reliability. One currently preferred definition is a protein which inhibits milk secretion by lactating cows and which is present in the eighth significant peak (6B) when a nominally 10-30 KDa fraction of the whey proteins of the milk is reso
REFERENCES:
Prentice et al. Biochemical Society Transactions Apr. 1989 p. 122.
Wilde et al. Biochimica Biophysica Acta. 992: 315-319 1989. I.
Hill et al. Can Inst. Food Scien. Tech. 19: 5 227-230 1986.
Wilde et al II, Quarty Journal of Experimental Physiology 73 391-397 1988.
Goding Monoclonal Antibod. Principles and Practice, 1986, Academic Press p. 59.
Addey Caroline V. P.
Peaker Malcolm
Wilde Colin J.
British Technology Group Ltd
Feisee Lila
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