Organic compounds -- part of the class 532-570 series – Organic compounds – Carbohydrates or derivatives
Patent
1989-05-19
1993-03-23
Martinell, James
Organic compounds -- part of the class 532-570 series
Organic compounds
Carbohydrates or derivatives
435 691, 4351723, 4353201, 935 6, C12N 1511, C12N 1500
Patent
active
051965232
ABSTRACT:
The regulatory sequence for a glucose regulated protein has been isolated. When fused into suitable expression vectors within reading relationship of a desired polypeptide DNA sequence, the resulting hybrid gene can be induced to high levels by glucose starvation, or calcium shock, or by denaturing reagents such as .beta.-mercaptoethanol, in a variety of host systems. Further, the hybrid gene can also be regulated by temperature when it is introduced into temperature-sensitive host systems such as the temperature-sensitive mutant of Chinese hamster cells or other systems having a compatible mutation.
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Martinell James
The University of Southern California
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