Control of flow cytometer having vacuum fluidics

Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample

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422 67, 422 681, 7386483, 436 63, G01N 2111, G01N 2708

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active

053955889

ABSTRACT:
The present invention relates to a system for controlling the movement or transport of objects such as cells and other particles in a moving liquid stream. More particularly the invention relates to a system including a vacuum pump which pulls the sheath fluid from an open supply reservoir through a flow cell assembly where cell analysis occurs and discharges the flow cell effluent to an open waste reservoir. A pressure drop is created through the conduit leading from the supply reservoir to the flow cell which aspirates the cell suspension from an open sample vessel into and through the flow cell. The vacuum is regulated by controlling the electric power applied to the vacuum pump motor. Starting, stopping, reverse flushing, and draining of the flow cell assembly are controlled by programming the operation of four electric solenoid valves in fluid communication with the flow cell inlet and outlet passages, the operation of a reverse flush pump and, the operation of an electrically controlled tube lifter. A deaerator is provided to remove some of the air dissolved in the sheath fluid provided to the flow cell assembly to prevent the release of air and formation of adherent air bubbles in sensitive areas of the flow cell assembly.

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Perry et al. "Membrane Processes" in Chemical Engineer's Handbook, McGraw Hill, N.Y. pp. 17-34 to 17-43.
Principles of Flow Cytometry--J. L. Haynes--Cytometry Supplement 3:7-17 (1988) pp. 7-17.

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