Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1981-11-23
1986-04-01
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 68, 435194, 435240, 435317, 935 6, 935 34, 935 36, 935 53, 935 70, 935111, C12N 1500, C12N 500, C12N 100, C12P 2100
Patent
active
045798214
ABSTRACT:
The transcription of DNA sequences in living cells is subjected to external regulation by incorporation of promoter/regulator DNA sequences responsive to metals and/or steroids. More particularly, regulation of the transcription of selected exogenous DNA sequences incorporated into eukaryotic host cells is facilitated by operative association (e.g., fusion) of the selected sequence to a promoter/regulator DNA sequence which is positively or negatively responsive to environmental variation in the concentration of heavy metal ions and/or steroid hormones. As an example, a structural gene for thymidine kinase from herpes simplex virus, fused to the promoter/regulator DNA sequence of a mouse metallothionein-I gene and incorporated on a suitable DNA plasmid vector, is introduced into mouse embryos and stably incorporated therein. Gene expression in differentiated cells of adult mice resulting from the embryos is subsequently regulatable by administration of heavy metals such as cadmium or steroid hormones such as the glucocorticoid, dexamethasone.
REFERENCES:
Durnam et al., Isolation and Characterization of the Mouse Metallothionein-I Gene, Proc. Natl. Acad. Sci. vol. 77, 80, pp. 6511-6515.
Wagner et al., The Human Beta Globin Gene and a Functional Viral Thymidine Kinase Gene in Developing Mice, Proc. Natl. Acad. Sci. v78.
Watson, Molecular Biology of the Gene, 1977, 3rd Ed. 1981, pp. 5016-5020.
Benjamin, Inc., London, pp. 561-562.
Karin, Regulation of Metallothionein Synthesis in HELA Cells, Diss. Abstr. Int., B 1980, v40(9) 4084 (date of Diss, 1979).
Brinster Ralph L.
Palmiter Richard D.
Tanenholtz Alvin E.
University Patents Inc.
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