Continuous spectrophotometric assay of microbial cellulase

Details Continuous spectrophotometric assay of microbial cellulase Continuous spectrophotometric assay of microbial cellulase

1982-01-25

1983-09-06

Hruskoci, Peter A.

Chemistry: molecular biology and microbiology

Measuring or testing process involving enzymes or...

Involving hydrolase

435 29, 435 34, 435209, 435808, C12Q 134

Patent

active

044030320

ABSTRACT:
A continuous spectrophotometric assay monitors the release of colored cellulose oligosaccharides during the cellulase catalyzed hydrolysis of dyed crystalline cellulose preparations. An anthraquinone derivative, Remazol Brilliant Blue R-Salt, is used to uniformly dye a variety of cellulose biopolymers without altering their respective polysaccharide conformations. The increase in dye absorbance of a cellulose filtrate is a direct measure of the total concentration of carbohydrates formed and the cellulase activity. The continuous cellulase assay has specific utility in the study of cellulase kinetics and in comparing the cellulase activities of different microorganisms.

REFERENCES:
patent: 3676303 (1972-07-01), Ingelman et al.
patent: 4066509 (1978-01-01), Ceska
Huang et al., "Sensitive Assay for Cellulase and Dextranase," Anal. Biochem., 73, 1976, pp. 369-377.
The Condensed Chemical Dictionary, 8th Edition, 1971, pp. 85, 214, 780.
Ng et al., "A Continuous Spectro. Assay for the Deter. of Cellulase Solubilizing Activity," Anal. Biochem., 103, 1980, pp. 42-50.
Ghose et al., "Measure of Cellulase Activity," IUPAC Commission on Biotechnology, Mar. 1981, pp. 1-113.

Affiliated with

Also associated with

Rating

Continuous spectrophotometric assay of microbial cellulase does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Continuous spectrophotometric assay of microbial cellulase, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Continuous spectrophotometric assay of microbial cellulase will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-1592862