Continuous production of ethanol by use of respiration deficient

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing oxygen-containing organic compound

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435255, 435940, C12P 706, C12N 116

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active

045671451

ABSTRACT:
This invention provides a process for producing ethanol from a D-sugar in a continuous aerobic environment using a flocculant respiration-deficient mutant of Saccharomyces uvarum in a single stage fermentor with a cell settling tank and cell recycle, at a productivity of more than 50 grams ethanol per liter fermentor volume per hour. The process comprises (a) innoculating a fermentation zone with a respiration-deficient mutant of Saccharomyces uvarum; (b) feeding a mixture of a D-sugar, a nitrogen source, a vitamin source and a mineral source into the fermentation zone in the presence of oxygen, and (c) fermenting the D-sugar mixture for a sufficiently long period of time to yield an ethanol product. In the process, the yeast are allowed to settle for a sufficiently long period of time and recycled to the fermentation zone to increase ethanol productivity. The preferred D-sugar that may be used in the present process to produce ethanol, is D-glucose.

REFERENCES:
patent: 4368268 (1983-01-01), Gong
Righelato et al., Chem. Abst., vol. 94 (1981), p. 101,297y.
Faust et al., Chem. Abst., vol. 94 (1981), p. 207,162r.

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