Continuous process for the enzymatic preparation of isomaltulose

Details Continuous process for the enzymatic preparation of isomaltulose Continuous process for the enzymatic preparation of isomaltulose

1986-04-23

1989-08-15

Wax, Robert A.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing compound containing saccharide radical

435 97, 435100, 435174, 435177, 435178, 435179, 435180, 435181, 435182, 435800, 435813, 435815, 435819, 435847, 435880, C12P 1924

Patent

active

048574619

ABSTRACT:
The present invention relates to a continuous process for the enzymatic preparation of isomaltulose. A periplasmatic sucrose-mutase is produced by fermentation of microorganisms which form sucrose-mutase. The cell-free crude enzyme extract is prepared by digestion of the cells and by cross-flow microfiltration. In a single-stage, simultaneous purification and immobilization of the sucrose-mutase from the cell-free crude extract conditioned by diafiltration is obtained by selective bonding to an anionizable carrier matrix. Direct conversion of sucrose into isomaltulose is produced by the sucrose-mutase bonded to the anionizable carrier matrix, preferably in cartridge or cartouche form.

REFERENCES:
patent: 4359531 (1982-11-01), Bucke et al.
patent: 4390627 (1983-06-01), Lantero
patent: 4525457 (1985-06-01), Sakata et al.
patent: 4663163 (1987-05-01), Hou et al.
Cheethan, P. S. J., "Principles of Industrial Enzymology: Basis of Utilization of Soluble and Immobilized Enzymes in Industrial Processes", in: Wiseman, A., Handbook of Enzyme Biotechnology, (Chichester, England, 1985), Ellis Horwood Ltd., 2nd ed., pp. 79-86.
S. Schmidt-Berg-Lorenz, W. Mauch, Z Zuckerindustrie 14, 625-627 (1964).
Technische Rundschau, Band 77, Nr. 38, 17. Sep. 1985, Seiten 38,39,42,43,Bern, CH; U. Beerstecher: "Forschung und Innovation auf der Achema 1985", *Figur 1*.

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