Continuous process for producing 2-keto-L-gulonic acid

Details Continuous process for producing 2-keto-L-gulonic acid Continuous process for producing 2-keto-L-gulonic acid

1999-07-13

2001-05-29

Prats, Francisco (Department: 1651)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Preparing oxygen-containing organic compound

C435S042000, C435S170000, C435S252100

Reexamination Certificate

active

06238897

ABSTRACT:

SUMMARY
The present invention relates to a continuous fermentation process for the manufacture of 2-keto-L-gulonic acid (2-KGA) from D-sorbitol. 2-KGA is a valuable intermediate for the production of ascorbic acid (vitamin C).
BACKGROUND OF THE INVENTION
Processes for the manufacture of 2-KGA from D-sorbitol are known. For example, EP 0 518 136 A2 discloses a fermentation process utilizing a mixed microorganism culture, whereby D-sorbitol is oxidized to L-sorbose with a microorganism belonging to the genus Gluconobacter or Acetobacter, e.g.,
Glitcoitobacter suboxydans
IFO 3291, and the L-sorbose is in turn converted to 2-KGA by fermentation with the microorganism strain DSM 4025, also known as
Gluconobacter oxydans
DSM 4025. In this process both microorganisms coexist in the fermentation medium during at least part of the entire cultivation period. It has been found, however, that the yield in the process disclosed in EP 0 518 136 A2 is unsatisfactory when the process is carried out in continuous manner.


REFERENCES:
patent: 5312741 (1994-05-01), Hoshino et al.
patent: 0 518 136 A2 (1993-01-01), None
patent: 1 120 249 (1968-07-01), None
patent: WO 98/17819 (1998-04-01), None
Campbell, “Biology,” The Benjamin/Cummings Publishing Company, Inc., California, pp. 1113-1114, 1993.*
Crueger et al. “Biotechnology: A Textbook of Industrial Microbiology,” Sinauer Associates, Inc. Massachusetts, pp. 67, 68, 97, 98, 1989.*
Tatsuo Hoshino, et al., Metabolic Pathway for 2-Keto-L-gulonic Acid Formation inGluconobacter melanogenusIFO 0323, Agri. Biol. Chem., vol. 54 (5), 1211-1218 (1990).
Teruhide Sugisawa, et al., Microbial Production of 2-Keto-L-Gulonic Acid from L-Sorbose and D-Sorbitol byGluconobacter melanogenus, Agri. Boil. Chem., vol. 54 (5), 1201-1209 (1990).
Tatsuo Hoshino, et al., Isolation and Characterization of NAD(P)-Dependent L-Sorbosone Dehydrogenase fromGluconobacter melanogenusUV10, Agri. Biol. Chem., vol. 55 (3), 655-670 (1991).
Teruhide Sugisawa, et al., Purification and Properties of NADPH-Linked L-Sorbose Reductase forGluconobacter melanogenusN44-1, Agri. Biol. Chem., vol. 55 (8), 2043-2049 (1991).
Chemical Abstracts, vol. 1186, No. 21, abstract No. 212895, accession No. XP-002119882 (1992).

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