Continuous-multi-step dilution process and apparatus, for the re

Chemistry: molecular biology and microbiology – Differentiated tissue or organ other than blood – per se – or... – Including perfusion; composition therefor

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435 11, A01N 100, A01N 102

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058798765

ABSTRACT:
The present invention is directed to a continuous, multi-step dilution process for producing tissue suitable for transplantation into a human from cryopreserved tissue. Cryopreserved tissue is subjected to a continuous flow of wash-out solution following a thawing or simultaneously thawing using the present continuous perfusion chamber. The present continuous perfusion chamber may be rigid or deformable and has an inlet port and an outlet port.

REFERENCES:
patent: 5700632 (1997-12-01), Critser et al.
Toledo-Pereyra, LH. Organ Freezing. in "Basic Concepts of Organ Procurement, Perfusion, and Preservation for Transplantation." Toledo-Pereyra, editor. Academic Press, New York. pp. 159-181., 1982.

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