Continuous cell dispersion, cultivation and substance recovery p

Details Continuous cell dispersion, cultivation and substance recovery p Continuous cell dispersion, cultivation and substance recovery p

1987-11-27

1990-05-01

Richman, Barry S.

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

43524025, 43524027, 435803, 435813, 435 7021, 435 703, C12P 300, C12P 2100, C12N 502

Patent

active

049217925

ABSTRACT:
A system for continuous production and removal of a biological substance from a dispersed cell culture. The system comprises a reactor adapted to maintain the cell culture under incubating conditions and means for pumping limited volumes of the culture fluid from the reactor and through an external substance separation device adapted to extract the substance in a limited time on a continuous basis without collection of, or damage to, the dispersed cells. Less than 5% of the total culture fluid volume is outside the reactor (Volume outside or Vo) at any time and a given dispersed cell is outside the protective environment of the reactor for less than about two minutes, as expressed by the following relationship, ##EQU1## In preferred embodiments the external device comprises a plurality of substance-extracting, porous, hollow fibers through which the culture fluid, substance, and dispersed cells are continuously passed. In other preferred embodiments, separate means for continuously dialyzing the culture fluid prior to its return to the reactor are provided.

REFERENCES:
patent: 4722902 (1988-02-01), Harm et al.
patent: 4780205 (1988-10-01), Murakami et al.
patent: 4806484 (1989-02-01), Petrossian et al.

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