Chemistry: molecular biology and microbiology – Spore forming or isolating process
Patent
1978-06-26
1980-01-22
Rosen, Sam
Chemistry: molecular biology and microbiology
Spore forming or isolating process
435286, C12K 900
Patent
active
041849167
ABSTRACT:
A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without cell disruption. The apparatus comprises a hollow flask assembly having a hollow shaft means journaled for rotation in a stationary sleeve sub-assembly and a filter unit suspended downwardly therefrom, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means and adapted for coaxial rotation with said shaft means.
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Feder Joseph
Kimes Richard C.
Tolbert William R.
Meyer Scott J.
Monsanto Company
Rosen Sam
Williams, Jr. James W.
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