Continuous cell culture method and apparatus

Details Continuous cell culture method and apparatus Continuous cell culture method and apparatus

1978-03-06

1979-12-11

Jones, Raymond N.

Chemistry: molecular biology and microbiology

Spore forming or isolating process

435286, 195143, 435286, C12K 900

Patent

active

041782097

ABSTRACT:
A cell culture system and apparatus is provided for the suspension culturing of mammalian cells in which fresh media can be added and spent medium filtered and withdrawn on a continuous or semi-continuous basis without any substantial cell disruption and clogging of the filter with cell debris. The apparatus comprises a hollow flask assembly having a stationary hollow shaft means and filter unit suspended downwardly from the top thereof, and a rotatable agitator means concentrically disposed about said filter unit, said filter unit being in fluid communication with the top opening of the flask through the interior of said hollow shaft means.

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