Chemical apparatus and process disinfecting – deodorizing – preser – Analyzer – structured indicator – or manipulative laboratory... – Means for analyzing liquid or solid sample
Patent
1995-05-10
1996-10-15
Warden, Jill
Chemical apparatus and process disinfecting, deodorizing, preser
Analyzer, structured indicator, or manipulative laboratory...
Means for analyzing liquid or solid sample
422 69, 422 70, 422 81, 422101, 422103, 422107, 422116, 436 89, 436 94, 436177, 436178, G01N 3368
Patent
active
055651712
ABSTRACT:
A novel reactor for reacting and subsequently analyzing sub-picomole quantities of a sample organic molecule. The reactor includes a continuous capillary connected between two valves that control fluid flow in the capillary. One part of the capillary forms a reaction chamber where the sample may be immobilized for subsequent reaction with reagents supplied through the valves. Another part of the capillary passes through or terminates in the detector portion of an analyzer such as an electrophoresis apparatus, liquid chromatographic apparatus or mass spectrometer. The apparatus may form a peptide or protein sequencer for carrying out the Edman degradation reaction and analyzing the reaction product produced by the reaction. The protein or peptide sequencer includes a reaction chamber for carrying out coupling and cleavage on a peptide or protein to produce derivatized amino acid residue, a conversion chamber for carrying out conversion and producing a converted amino acid residue and an analyzer for identifying the converted amino acid residue. The reaction chamber may be contained within one arm of a capillary and the conversion chamber is located in another arm of the capillary. An electrophoresis length of capillary is directly capillary coupled to the conversion chamber to allow electrophoresis separation of the converted amino acid residue as it leaves the conversion chamber. Identification of the converted amino acid residue takes place at one end of the electrophoresis length of the capillary.
REFERENCES:
patent: 4603114 (1986-07-01), Hood et al.
patent: 4610847 (1986-09-01), Hood et al.
patent: 4704256 (1987-11-01), Hood et al.
patent: 4935624 (1990-06-01), Henion et al.
Nolan et al., Subfemtomole Detection Limit for Amino Acid Determination with Laser-Induced Crossed-Beam Thermal Lens Detection; Analytical Chemistry, vol. 59, No. 23, Dec. 1, 1987; pp. 2803-2805.
Green et al., Design of a Variable Wavelength UV Absorption Detector For On-Column Detection in Capillary Electrophoresis and Comparison of its Performance to a Fixed Wavelength UV Absorption Detector; Journal of Liquid Chromatography, vol. 12, No. 13; pp. 2527-2561, 1989.
Needleman, Ed., Protein Sequence Determination, A Source book of Methods and Techniques; Second Revised Edition and Enlarged Edition, Chapter 8, pp. 232-385; 1985.
Smith, Automated Synthesis and Sequence Analysis of Biological Macromolecules; Analytical Chemistry, vol. 60, No. 6; pp. 381A-390A; Mar. 15, 1988.
Capillary electrophoresis, Instrumentation '91; C&EN; pp. 28, 35, Mar. 18, 1991.
Kent et al., Overview: Approaches to Sub-Picomole Protein Sequencing; Bio Techniques, vol. 5, No. 4; pp. 314-321, 1987.
Hewick et al., A Gas-Liquid-Solid Phase Peptide and Protein Sequenator; The Journal of Biological Chemistry, vol. 256, No. 15; pp. 7990-7997, Aug. 10, 1981.
Bhown, Ed., Protein/Peptide Sequence Analysis: Current Methodologies; Chapter 5, Gas Phase Sequence Analysis of Proteins/Peptides; CRC Press, Inc.; pp. 87-117; 1988.
Bornhop et al., Simultaneous Laser-Based Refractive Index and Absorbance Determinations within Micrometer Diameter Capillary Tubes; Analytical Chemistry, vol. 59, No. 13; pp. 1632-1636; Jul. 1, 1987.
Long et al., Thermal Lens Technique: A New Method of Absorption, Spectroscopy; Science, vol. 191; pp. 183-185, Jan. 16, 1976.
Liang et al., Covalent Immobilization of Proteins and Peptides for Solid-Phase Sequencing Using Prepackaed Capillary Columns; Analytical Biochemistry, vol. 188; pp. 366-373; 1990.
Cheng et al, Subattomole Amino Acid Analysis by Capillary Zone Electrophoresis and Laser-Induced Fluorescence, Science, vol. 242, pp. 562-564, 1988.
Swerdlow et al., Three DNA Sequencing Methods Using Capillary Gel Electrophoresis and Laser-Induced Fluorescence, Analytical Chemistry, vol. 63, No. 24, 2835-2841; Dec. 15, 1991.
Yu et al., Attomole Amino Acid Determination by Capillary Zone Electrophoresis with Thermooptical Absorbance Detection; Analytical Chemistry, vol. 61, No. 1; pp. 37-40, Jan. 1, 1989.
Waldron et al., Sub-Femtomole Determination of Phenylthiohydantoin-Amino Acids: Capillary Electrophoresis and Thermooptical Detection, Reprinted from Analytical Chemistry, vol. 64, No. 13, pp. 1396-1399, Jul. 1, 1992.
Drossman et al., High-Speed Separations of DNA Sequencing Reactions by Capillary Electrophoresis; Analytical Chemistry, vol. 62, pp. 900-903, 1990.
Maeda et al., Reaction of Fluorescein-Isothiocyanate with Proteins and Amino Acids, part I; The Journal of Biochemistry, vol. 65, No. 5, pp. 777-783, 1969.
Kawauchi et al., Reaction of Fluorescein-Isothiocyanate with Proteins and Amino Acids, part II; The Journal of Biochemistry, vol. 66, No. 6, pp. 783-789, 1969.
Muramoto et al., The Application of Fluorescein Isothiocyanate and High-Performance Liquid Chromatography for the Microsequencing of Proteins and Peptides; Analytical Biochemistry, vol. 141, pp. 446-450, 1984.
Knoll, Estimation of the Limit of Detection in Chromatography; Journal of Chromatographic Science, vol. 23, 4 pages, Sep. 1985.
Tempst et al., Examination of Automated Polypeptide Sequencing Using Standard Phenyl Isothiocyanate Reagent and Subpicomole High-Performance Liquid Chromatographic Analysis; Analytical Biochemistry, vol. 183, pp. 290-300, 1989.
Cohen et al., High-Performance Capillary Electrophoretic Separation of Bases, Nucleosides, and Oligonucleotides: Retention Manipulation via Micellar Solutions and Metal Additives; Analytical Chemistry, vol. 59, No. 7, pp. 1021-1027, Apr. 1, 1987.
Liu et al., Capillary electrophoretic separations of peptides using micelleforming compounds and cyclodextrins as additives; Journal of Chromatography, vol. 519, pp. 189-197, 1990.
Terabe et al., Electrokinetic Separations with Micellar Solutions and Open-Tubular Capillaries; Analytical Chemistry, vol. 56, No. 1, pp. 111-113, 1984.
Mikkers et al., Concentration Distributions In Free Zone Electrophoresis; Journal of Chromatography, vol. 169, pp. 1-10, 1979.
Loo et al., Primary Sequence Information from Intact Proteins by Electrospray Ionization Tandem Mass Spectrometry; Science, vol. 248, pp. 201-204, Apr. 13, 1990.
Jeno et al., CRC: Laboratory Methodology in Biochemistry Amino Acid Analysis and Protein Sequencing; Chapter 5, Application of 4-N, N-Demethylaminoazobenzene-4'-Isothiocyanate (Dabitc) to the Structure Determination of Peptides and Proteins; pp. 63-81.
Aebersold et al., Covalent Immobilization of Proteins for High-Sensitivity Sequence Analysis: Electroblotting onto Chemically Activated Glass from Sodium Dodecyl Sulfate-Polyacrylamide Gels; Biochemistry, vol. 27, pp. 6860-6867, 1988.
McGuffin et al., Aids for Analytical Chemists: Nanoliter Injection System for Microcolumn Liquid Chromatography; Analytical Chemistry, vol. 55, No. 3; pp. 580-583, Mar. 1983.
Kettler et al., Pulsed-Laser Photothermal Refraction Detection in Capillary Liquid Chromatography; Analytical Chemistry, vol. 59, No. 13; pp. 1733-1736, Jul. 1, 1987.
Otsuka et al., Electokinetic Chromatography with Micellar Solutions Separation of Phenylthiohydantoin-Amino Acids; Chromsymp. 617, pp. 219-226, .COPYRGT. 1985 Elsevier Science Publishers, B.V.
Swerdlow et al., Capillary gel electrophoresis for DNA sequencing, Laser-induced fluorescence detection with the sheath flow cuvette; Journal of Chromatography, vol. 516, pp. 62-67, 1990.
Jorgensen et al., Capillary Zone Electrophoresis; Science, vol. 222, pp. 266-272; Oct. 21, 1983.
Wu et al., High-Sensitivity Fluorescence Detector for Fluorescein Isothiocyanate Derivatives of Amino Acids Separated by Capillary Zone Electrophoresis; Journal of Chromatography, vol. 480, pp. 141-155, 1989.
Rohlicek et al., Simple Apparatus for Capillary Zone Electrophoresis and its Application to Protein Analysis; Journal of Chromatography, vol. 494, pp. 87-98, 1989.
Sweedler et al., Fluorescence Detection in Capillary Zone Electrophoresis Using a Charge-Coupled Device with Time-Delayed Integration; Analytical Chemistry, vol. 63, No. 5; pp. 496-502, Mar. 1, 1991.
Monnig et al., On-Column Sample Gating for High-Speed Capillary Zone Electrophoresis; Analytical Chemi
Dovichi Norman J.
Waldron Karen C.
Governors of the University of Alberta
Pyon Harold Y.
Warden Jill
LandOfFree
Continuous biochemical reactor for analysis of sub-picomole quan does not yet have a rating. At this time, there are no reviews or comments for this patent.
If you have personal experience with Continuous biochemical reactor for analysis of sub-picomole quan, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Continuous biochemical reactor for analysis of sub-picomole quan will most certainly appreciate the feedback.
Profile ID: LFUS-PAI-O-1243740