Continous fluorometric assay for detecting nucleic acid cleavage

Chemistry: molecular biology and microbiology – Measuring or testing process involving enzymes or... – Involving nucleic acid

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435 18, 435 912, 536 243, C12Q 168, C12P 1934, C07H 2104

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057631816

ABSTRACT:
A method of detecting an enzyme-mediated DNA cleavage reaction in a fluorometric assay is provided. The method can be used to detect DNA cleavage caused by restriction endonucleases, retroviral integrase enzymes, DNases, RNases, or enzymes utilized in other strand separating processes in molecular biology.

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