Containers for culturing and testing of vertebrate lenses

Details Containers for culturing and testing of vertebrate lenses Containers for culturing and testing of vertebrate lenses

1986-03-27

1989-09-12

Richman, Barry S.

Chemistry: molecular biology and microbiology

Apparatus

Differentiated tissue perfusion or preservation apparatus

435285, 435287, 435297, 435298, 435299, 435300, 435301, 422102, C12M 300

Patent

active

048659851

ABSTRACT:
A container for holding and culturing a lens is disclosed, comprising a transparent base member, a transparent lens carrier supported laterally in the base member and having a central aperture, and a transparent cover engaging the base member for enclosing the lens carrier within the container. The base is circular in top view, and in sectional elevation is in the form of a flanged U-shape, forming a well. The lens carrier is a circular plate with a central aperture. The lip of the aperture tapers downwardly in order to support the outer rim of the lens to be tested. The cover has an annular flange and a raised center section with angled sidewalls. The sidewalls have two flat sections offset from each other by 90 degrees, which act as viewing ports. The lens to be studied is centered in the aperture in the bottom of the lens carrier. The lens carrier is ordinarily provided with passage holes to permit the free passage of the culture medium from one side of the lens to the other. However, a lens carrier without such holes may be used if it is desired to expose the anterior and posterior lens surfaces to separate media.

REFERENCES:
patent: 2942520 (1960-06-01), Rose
patent: 3128902 (1964-04-01), Barnum
patent: 4308351 (1981-12-01), Leighton et al.
patent: 4358425 (1982-11-01), Finney et al.
patent: 4435508 (1984-03-01), Gabridge
patent: 4446234 (1984-05-01), Russo et al.
patent: 4652429 (1987-03-01), Konrad
patent: 4668633 (1987-05-01), Walton
"Spherical Aberration of the Crystalline Lens" J. G. Sivak and R. O. Kreuzer, Vision Res. vol. 23, pp. 59-70, 1983.
"Aging and the Optical Quality of the Rat Crystalline Lens" J. G. Sivak and A. Dovrat, Investigative Opthalmology & Visual Science, vol. 24, pp. 1162-1166, Sep. 1983.

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