Chemistry: molecular biology and microbiology – Apparatus – Differentiated tissue perfusion or preservation apparatus
Patent
1989-08-28
1992-01-28
Jones, Larry
Chemistry: molecular biology and microbiology
Apparatus
Differentiated tissue perfusion or preservation apparatus
435296, 206223, 215 1C, C12M 300
Patent
active
050843931
DESCRIPTION:
BRIEF SUMMARY
DESCRIPTION
1. Technical Field
My invention relates to containers for biological cultures for example for growing cells.
Containers for biological cultures are formed for example as flasks or roller bottles. They are to be filled with a nutrient medium, and cells are then grown in the containers. A known container of this type is as a rule a vessel of a round, rectangular or other cross-section with substantially smooth inner walls and bottom. The output of the container depends on a surface available for growing of cells.
2. Background Art
For increasing the area of cell growing it is known to introduce into the container additional surfaces, to form the container by a plurality of flat dishes which are stackable over one another, and produce additional formations in the container. In one of the known containers of this type disclosed in U.S. Pat. No. 4,824,787 a plurality of corrugations are provided, which increase the area of growing cells. The increase of the area achieved in this construction is however very limited, and it is to be understood that it is desired to increase further the area for growing cells and therefore to increase the output of the containers for biological cultures.
DISCLOSURE OF INVENTION
In accordance with the present invention, I provide a container for a biological culture, particularly for growing cells, which has a wall limiting an inner chamber with an area for growing cells in a solution, wherein the wall has at least one wall portion formed as a brush with a plurality of bristle-shaped projections extending into said area for supporting cells.
My improved container for biological culture possesses the advantage that the bristle-shaped projections provide a considerably increased surface for supporting cells than any other formations known in the art. Each bristle is connected with the wall only at its end, while the opposite end of the bristle as well as its side surface and surfaces at all sides are available for supporting the cells.
The brush-like wall portion can be a portion near which a solution is accumulated, so that the bristle-shaped projections extend from this wall portion and are completely covered with the solution. On the other hand the brush-like wall portion can be opposite to a wall portion near which a solution is accumulated, so that the bristle-shaped projections extend toward the wall at which the solution is accumulated. The bristle-shaped projections always have a height which is greater than their thickness, and can be rigid or flexible. They can have different shapes which are suitable for growing of cells on them.
In a container formed in accordance with the present invention as a roller bottle the cells grow not only in the area located in the region of the main body of nutrient solution. Since the projections are bristle shaped, they retain the nutrient solution even when they are withdrawn from its main body during rotation of the bottle. Thereby the cells also grow in the regions outside the main body of the solution on the projections on which the solution is retained and slowly flows down from them.
BRIEF DESCRIPTION OF DRAWINGS
The details of my invention will be described in connection with the accompanying drawings, in which FIGS. 1, 2, 3 are a side view at transverse section and a partial view of a bottom of a container for biological culture formed as a flask; FIGS. 4, 5 and 6 are a side view, a section and a view of an inner surface of the container for biological culture formed as a roller bottle; FIGS. 7-18 are schematic views showing bristle-shaped projections of the container for biological culture, having different shapes; FIGS. 19, 20 and 21, 22 show a detailed plan view and a detailed side view of two further modifications of the bristle-shaped projections for a flask-type container for biological cultures; FIGS. 23 and 24 are views showing two further modifications of the bristle-shaped projections for the container formed as a roller bottle; FIGS. 25-28 are schematic views showing a container formed as a flask in acc
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Jones Larry
Zborovsky Ilya
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