Constructs for gene expression analysis

Chemistry: molecular biology and microbiology – Vector – per se

Reexamination Certificate

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C435S069700, C435S455000, C435S419000, C435S325000, C536S023400, C536S024100

Reexamination Certificate

active

10658093

ABSTRACT:
The present invention relates generally to constructs and their use in gene expression or gene regulation assays. More particularly, the present invention provides expression vectors and/or reporter vectors providing kinetics of protein expression with improved temporal correlation to promoter activity. Even more particularly, the invention provides expression vectors comprising a transcribable polynucleotide which comprises a sequence of nucleotides encoding a RNA element that modulates the stability of a transcript corresponding to the transcribable polynucleotide. The present invention provides, inter alia, novel vectors, useful for identifying and analysing cis- and trans-acting regulatory sequences/factors as well as vectors and genetically modified cell lines or organisms that are particularly useful for drug screening and drug discovery.

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