Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Recombinant dna technique included in method of making a...
Reexamination Certificate
2005-01-18
2005-01-18
Ketter, James (Department: 1636)
Chemistry: molecular biology and microbiology
Micro-organism, tissue cell culture or enzyme using process...
Recombinant dna technique included in method of making a...
C435S071100, C435S071200, C435S488000, C435S252330
Reexamination Certificate
active
06844169
ABSTRACT:
The invention concerns a novel construct for expressing a gene coding for a recombinant protein of interest placed under the control of the Ptrp tryptophan operon promoter in a procaryotic host cell. The invention is characterised in that the construct comprises a nucleic sequence capable of inactivating the gene coding for a TnaA tryptophanase when said nucleic sequence is introduced in said host cell. The invention also concerns vectors containing said construct and host cells transformed by said vectors. The invention further concerns methods for producing said recombinant proteins using said novel constructs.
REFERENCES:
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Yanofsky et al. Some novel transcription attenuation mechanisms used by bacteria Biochimie 1996 78, 1017-1024.*
Kamath, et al. Characterization of the Tryptophanase Operon ofProteus vulgaris. (1992) The Journal of Biological Chemistry 267:19978-85.
Phillips, et al. Evidence That Cysteine 298 Is in the Active Site of Tryptophan Indole-Iyase (1989) The Journal of Biological Chemistry 264:10627-32.
Yansura, D. et al., “Use ofEscherica colitrp promoter for direct expression of proteins”, Methods in Enzymology, vol. 185, pp 54-60, 1990.
Yanofsky, C. et al., “Some novel trnascription attenuation mechanisms used by bacteria”, Biochime, vol. 78, No. 11-12, pp 1017-1024, 1996.
Warne, S. et al., “Use of a modifiedEscherichia colitrpR gene to obtain tight regulation of high-copy-No. expression vectors”, GENE, vol. 46, pp 103-112, 1986.
Bonnefoy Jean-Yves
Chevalet Laurent
Ngoc Nguyen Thien
Robert Alain
Katcheves Konstantina
Ketter James
Pierre Fabre Medicament
The Firm of Mueschen and Sage
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