Constructs and cells for production of small peptides

Details Constructs and cells for production of small peptides Constructs and cells for production of small peptides

2007-06-12

2007-06-12

Nickol, Gary B. (Department: 1646)

Chemistry: molecular biology and microbiology

Micro-organism, tissue cell culture or enzyme using process...

Recombinant dna technique included in method of making a...

C435S320100, C435S325000, C530S308000

Reexamination Certificate

active

09716166

ABSTRACT:
The present invention features a nucleic acid construct for expressing a product, e.g., a small peptide such as GLP-1. The construct includes a nucleic acid sequence encoding a signal peptide and a nucleic acid sequence which encodes the pro-region of somatostatin or a functional fragment thereof. The construct can further include a sequence encoding the small peptide or the construct can be used to express an endogenous genomic sequence encoding the small peptide. The present invention further features genetically engineered cells, and methods of using such constructs or cells.

REFERENCES:
patent: 5118666 (1992-06-01), Habener
patent: 5641670 (1997-06-01), Treco et al.
patent: 5891671 (1999-04-01), Suzuki et al.
patent: 6010883 (2000-01-01), Nagai et al.
patent: 6531124 (2003-03-01), Selden et al.
patent: 0 157 753 (1987-11-01), None
patent: 0 770 995 (1996-03-01), None
patent: 0 775 750 (1997-05-01), None
patent: WO 92/10576 (1992-06-01), None
patent: WO 93/09222 (1993-05-01), None
patent: WO 97/29180 (1997-08-01), None
patent: WO 98/37910 (1998-09-01), None
patent: WO 99/29336 (1999-06-01), None
Patel et al. Processing and intracellular targeting of prosomatostatin-derived peptides: the role of mammalian endoproteases. 1995, CIBA Foundation Sumposium, 190: 26-50.
Gerich et al. Prolonged intravenous and subcutaneous somatostatin in treatment of human diabetes. 1976, Diabetes, 25 (suppl. 1): 340.
Warren et al. Expression of preprosomatostatin in heterologous cells: biosynthesis, posttranslational processing, and secretion of mature somatatin, Cell, 1984, 39(3 Pt2): 547-55.
Schwartz, “The Processing of Peptide Precursors Proline-directed Arginyl Cleavage and Other Monobasic Processing Mechanisms”, FEBS Letters 200:1-10, 1986, XP-002164412.
Stroller et al., “The Propeptide of Preprosomatostatin Mediates Intracellular Transport and Secretion of α-Globin from Mammalian Cells”, Journal of Cell Biology 108:1647-1656, 1989, XP-000990902.
Argos et al., “Nucleotide and Amino Acid . . . ,” The Journal of Biological Chemistry, 258(14):8788-8793, 1983.
Chen et al., “The Propeptide of Anglerfish . . . ,” The Journal of Biological Chemistry, 270(31)18598-18605, 1995.
Fisher et al., “Multiple Neuropeptides Derived . . . ,” Cell, 54:813-822, 1988.
Moore et al., “Secretory Protein Targeting . . . ” The Journal of Cell Biology, 101:1773-1781, 1985.
Sevarino et al., “Amino-Terminal Sequences . . . ,” Cell, vol. 57:11-19, 1989.
Stoller et al., “The Propeptide of Preprosomatostatin . . . ,” The Journal of Cell Biology, 108:1647-1655, 1989.
GenBank Accession No. NP—001039, Jul. 26, 1999.
GenBank Accession No. AAD39138, Jun. 16, 1999.
GenBank Accession No. AAC04697, Apr. 20, 1999.
Stoller & Shields, “The role of paired basic amino acids in mediating proteolytic cleavage of prosomatostatin. Analysis using site-directed mutagenesis”, Journal of Biological Chemistry 264: pp. 6922-6928 (1989).
Danoff et al., “Intracellular degradation of prohormone-chloramphenicol-acetyl-transferase chimeras in a pre-lysosomal compartment”, Eur. J. Biochem. vol. 218, pp. 1063-1070 (1993).

Affiliated with

Also associated with

Rating

Constructs and cells for production of small peptides does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Constructs and cells for production of small peptides, we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Constructs and cells for production of small peptides will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-3877126