Construction of selectable shuttle cloning vectors for Thiobacil

Chemistry: molecular biology and microbiology – Micro-organism – tissue cell culture or enzyme using process... – Preparing compound containing saccharide radical

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4351723, 4353171, 935 22, 935 29, C12P 1934, C12N 1500, C12N 700

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047118490

ABSTRACT:
The plasmids pDR401 and pDR412 are disclosed which contain a selectable chloramphenicol resistance gene and which are able to replicate in both T. ferrooxidans and E. coli. A process for constructing the plasmids is also described.

REFERENCES:
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Rawlings, D. E. et al., "Expression of Thiobacillus ferrooxidans Origin of Replication in Escherichia coli", Journal of Bacteriology, vol. 158, No. 2, May, 1984, pp. 737-738.
Rawlings, D. E., et al., "Construction of arsenic-resistant Thiobacillus ferrooxidans recombinant plasmids and the expression of autotrophic plasmid genes in a heterotrophic cell-free system", Journal of Biotechnology, 1 (1984) 129-133.
Rawlings, D. C., et al., "Mobilization of Thiobacillus ferrooxidans plasmids among Escherichia coli Strains, Applied and Environmental Microbiology, vol. 49, No. 5, 1985, pp. 1323-1325.
Barros, M. E. C., et al., "Production and Regeneration of Thiobacillus ferrooxidans Spheroplasts, Applied and Environmental Microbiology, vol. 50, No. 3, Sep. 1985, pp. 721-723.

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