Construction of novel mutant microorganisms

Details Construction of novel mutant microorganisms Construction of novel mutant microorganisms

1983-09-15

1987-03-31

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435 68, 435317, 935 22, 935 56, C12N 1500, C12N 120

Patent

active

046543031

ABSTRACT:
This invention provides novel mutant strains of microorganisms (e.g., Pseudomonas putida Biotype A) which are capable of converting substrates such as toluene, p-xylene, catechol and 4-methylcatechol to 2-hydroxymuconic semialdehyde or substituted analog of 2-hydroxymuconic semialdehyde quantitatively by the meta (catechol 2,3-oxygenase) pathway.
No active 2-hydroxymuconic semialdehyde-metabolizing enzymes are induced in the microorganism, thereby permitting a 2-hydroxymuconic semialdehyde type metabolite to be produced and accumulated in a bioconversion medium containing the microorganism.

REFERENCES:
patent: 4355107 (1982-10-01), Maxwell
Yamazaki, H. et al., J. Bacteriol., vol. 156, pp. 327-337, 1983.
Ohmura, K. et al., Biochem. Biophys. Res. Comm., vol. 112, pp. 678-683, 1983.
Yang, M. et al., Nuc. Acids. Res., vol. 11, pp. 237-249, 1983.
Ohmura, K. et al., J. Biochem., vol. 95, pp. 87-93, 1984.
Davis et al., 1968, Can. J. Microbiol., v. 14(7), 1005-9.
Clarke (editor) et al., 1975, Genetics and Biochemistry of Pseudomonas, John Wiley & Sons, London, pp. 226-238.
Wigmore et al., 1974, "Pseudomonas putida Mutants Defective in the Metabolism of Products of meta Fission of Catechol . . . ", J. Bact., v 120, pp. 31-37.
Kellogg et al., 1981, "Plasmid-Assisted Molecular Breeding: New Technique for Enhanced Biodegradation of Persistent Toxic Chemicals", Science, v 214, pp. 1133-1135.

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