Construction of new .alpha.-galactosidase producing yeast strain

Details Construction of new .alpha.-galactosidase producing yeast strain Construction of new .alpha.-galactosidase producing yeast strain

1987-04-10

1991-10-08

Teskin, Robin L.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435208, 435254, 435255, 435256, 4353201, 435100, 435105, 435 91, 536 27, 935 27, 935 28, 935 37, 935 56, 935 69, 935 78, 935 82, C12N 1500, C12N 940, C12P 1939

Patent

active

050554018

ABSTRACT:
The objects of this invention are new Saccharomyces cerevisiae yeast strains into which .alpha.-galactosidase gene (MEL.sup.+) has been transferred by using recombinant DNA methods. Baker's and distiller's yeasts producing .alpha.-galactosidase, are utilizable in the corresponding industry, because they are able to utilize the raffinose present in molasses, which results in greater yield of yeast (or ethanol) and reduction or elimination of the costs associated with biological oxygen demand (B.O.D.) in the effluent from factories. The improved ability of brewer's yeasts to produce .alpha.-galactosidase provides a sensitive method for monitoring pasteurization of beer.
The new yeast strains prepared by using recombinant DNA methods produce more .alpha.-galactosidase than naturally occurring .alpha.-galactosidase producing yeast strains.
Also methods for marking yeast strains and for producing stable transformants of yeasts are presented.

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