Chemistry: molecular biology and microbiology – Enzyme – proenzyme; compositions thereof; process for... – Hydrolase
Reexamination Certificate
2011-05-31
2011-05-31
Wessendorf, Teresa D. (Department: 1639)
Chemistry: molecular biology and microbiology
Enzyme , proenzyme; compositions thereof; process for...
Hydrolase
C435S212000, C435S183000, C435S174000, C435S004000, C506S013000
Reexamination Certificate
active
07951572
ABSTRACT:
Disclosed herein is a gold nanoparticle (AuNP)-based peptide chip prepared by forming a monolayer of AuNPs onto a self-assembled monolayer constructed on a solid support, and then immobilizing a peptide on the AuNPs. The AuNPs can effectively amplify the mass signal of the peptide, thus making it possible to measure the mass change of the peptide in a simple and accurate manner. Also, when secondary ion mass spectrometric analysis (spectrum or imaging) is performed on the AuNP-based peptide chip, the activities of enzymes and related inhibitors can be effectively quantified. The disclosed invention enables various enzyme activities to be analyzed rapidly and accurately, and thus can provide an important method for disease diagnosis and new drug development through the elucidation of signaling and interaction mechanisms.
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Hong Mi-Young
Kim Hak-Sung
Kim Young-pil
Lee Dohoon
Lee Tae Geol
Korea Advanced Institute of Science and Technology
Korea Research Institute of Standards and Science
Staas & Halsey , LLP
Wessendorf Teresa D.
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