Chemistry: molecular biology and microbiology – Treatment of micro-organisms or enzymes with electrical or... – Modification of viruses
Patent
1980-10-01
1982-06-01
Tanenholtz, Alvin E.
Chemistry: molecular biology and microbiology
Treatment of micro-organisms or enzymes with electrical or...
Modification of viruses
435 91, 435253, 435317, 435820, 435849, C12N 1500, C12N 120, C12N 100, C12P 1934
Patent
active
043329004
ABSTRACT:
Novel chemical compounds, recombinant plasmids pUC1019 and pUC-1020, which are obtained by covalent linkage of ca. 4.2 kb BclI restriction endonuclease fragment of the Streptomyces espinosus plasmid pUC6 into the BamHI endonuclease site of the E. coli plasmid pBR322. Plasmid pUC1024 is obtained by restructuring plasmid pUC1019. These plasmids are useful as cloning vehicles in recombinant DNA work. For example, using DNA methodology, a desired gene, for example, the insulin gene, can be inserted into the plasmids and the resulting plasmids can then be transformed into a suitable host microbe which, upon culturing, produces the desired insulin.
REFERENCES:
patent: 4237224 (1980-12-01), Cohen et al.
patent: 4273875 (1981-06-01), Manis
Muray et al. in Recombinant DNA and Gentic Experimentation.
Morgan et al., (ed.) Pergamon Press, (1979), pp. 53-64.
Blattner et al., Science 196, 161, (1977).
Armstrong et al., Science 196, 172, (1977).
Bolivar, Gene 4, 121, (1978).
Highlander Sarah K.
Manis Jack J.
Martinell James
Saliwanchik Roman
Tanenholtz Alvin E.
The Upjohn Company
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