Construction of arsenic resistance vectors for Thiobacillus ferr

Details Construction of arsenic resistance vectors for Thiobacillus ferr Construction of arsenic resistance vectors for Thiobacillus ferr

1984-11-01

1988-05-31

Wiseman, Thomas G.

Chemistry: molecular biology and microbiology

Treatment of micro-organisms or enzymes with electrical or...

Modification of viruses

435253, 435320, 435849, 435281, 935 29, 935 56, C12N 1500, C12N 120, C12N 700

Patent

active

047481188

ABSTRACT:
A process for constructing the plasmids pDR416 and pDR420 which contain arsenic resistance genes and which are able to replicate in both T. ferroxidans and E. coli comprises preparing a recombinant of pBR325 or the like and a T. ferrooxidans plasmid, forming a deletion plasmid and inserting arsenic resistance genes e.g. from R46 by EcoR1 excision and ligation.

REFERENCES:
patent: 4340674 (1982-07-01), Monis et al.
Chemical Abstracts 95:3291t-1981, (Anthony M. Brown and Neil S. Willets, Plasmid, 1981, 5(2), 188-201).
Chemical Abstracts 101:145108e, 1984, (Nicolau, Claude et al.).
Kreft, J. et al., Mol. Gen. Genet., 1983, pp. 384-389.
Rawlings, D. E. et al., Abstract in French of International Symposium on Biohydrometallurgy, May 1, 1983, pp. 555-570.
Rawlings, D. E. et al., "Expression of Thiobacillus ferrooxidans Origin of Replication in Escherichia coli", Journal of Bacteriology, vol. 158, No. 2, May, 1984, pp. 737-738.
Rawlings, D. E. et al, "Construction of Arsenic-Resistant Thiobacillus ferrooxidans Recombinant Plasmids and the Expression of Autotrophic Plasmid Genes in a Heterotrophic Cell-Free System", Journal of Biotechnology, 1 (1984), 129-133.
Brown, Anthony M. C. et al., "A Physical and Genetic Map of the IncN Plasmid R46", Plasmid 5, 188-200 (1981).
Holmes et al., J. Bacteriol., 157(1): 324-326, 1984 (Jan.).
Martin et al., Con. J. Microbiol., 27:850-853, 1981.
Summers et al., In. Microbiology-1978, (ed. Schlessinger) A.S.M., 1978, pp. 128-131.

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