Construction of an IgG binding protein to facilitate downstream

Details Construction of an IgG binding protein to facilitate downstream Construction of an IgG binding protein to facilitate downstream

1990-10-09

1992-09-01

Lacey, David L.

Chemistry: molecular biology and microbiology

Micro-organism, per se ; compositions thereof; proces of...

Algae, media therefor

435 697, 4353701, 530417, C12N 1531, C12N 1562, C12N 1563

Patent

active

051438445

ABSTRACT:
A recombinant DNA fragment (Z) coding for an immunoglobulin G binding domain related to staphylococcal protein A. The methionine codon of the recombinant DNA fragment has been replaced with a codon of an amino acid residue other than methionine to enable expression of a methionine-free protein. The present invention is also directed to recombinant DNA sequence containing such recombinant DNAS fragment (Z) as well as a recombinant DNA molecule, plasmid vector and bacterial cell containing such recombinant DNAS fragment. Also disclosed is a process for cleaving a fused protein expressed in a biological system using said recombinant DNA fragment.

REFERENCES:
patent: 4769326 (1988-09-01), Rutter
J. Biol. Chem. 259;1695-1702, Feb. 10, 1984, Uhlen et al., Complete Sequence of the Staplylococcal Gene Encoding Protein A.
P.N.A.S. 82:7646-7650, Nov. 1985, Whitney et al. Detection of Neutral Amino Acid Substitutions in Proteins.

Affiliated with

Also associated with

Rating

Construction of an IgG binding protein to facilitate downstream does not yet have a rating. At this time, there are no reviews or comments for this patent.

If you have personal experience with Construction of an IgG binding protein to facilitate downstream , we encourage you to share that experience with our LandOfFree.com community. Your opinion is very important and Construction of an IgG binding protein to facilitate downstream will most certainly appreciate the feedback.

Rate now

Comments { 0 }

Profile ID: LFUS-PAI-O-767339